Dynein drives nuclear rotation during forward progression of motile fibroblasts.
Dynein drives nuclear rotation during forward progression of motile fibroblasts.
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DOI：
10.1242/jcs.033878
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发表时间：
2008-10-01
影响因子：
4
通讯作者：
Holzbaur EL
中科院分区：
文献类型：
--
作者：
Levy JR;Holzbaur EL
在定向细胞迁移过程中，细胞核的运动与细胞的前进相结合。微管运动细胞质动力蛋白是细胞极化和细胞运动所必需的。在这里，我们研究了动力蛋白促进定向迁移的机制。动力蛋白的敲低减缓了前缘的突出，导致核运动缺陷。在动力蛋白敲除的细胞中，细胞核的迁移速度减慢，细胞核错误地定位于运动细胞的后部。在对照细胞中，我们观察到损伤单层刺激了伤口边缘核旋转的显著诱导，速度高达8.5度/分钟。这些核旋转在动力蛋白敲低的细胞中被显著抑制。令人惊讶的是，中心体并不与细胞核一起旋转；相反，中心体稳定地位于细胞核和前缘之间。综上所述，这些结果表明动力蛋白通过两种方式促进迁移：(1)通过将微管+端系在皮层上来维持中心体的中心性；(2)通过直接对细胞核施加力来维持核的中心性。
During directed cell migration the movement of the nucleus is coupled to the forward progression of the cell. The microtubule motor cytoplasmic dynein is required for both cell polarization and cell motility. Here, we investigate the mechanism by which dynein contributes to directed migration. Knockdown of dynein slows protrusion of the leading edge and causes defects in nuclear movements. The velocity of nuclear migration was decreased in dynein knockdown cells, and nuclei were mislocalized to the rear of motile cells. In control cells, we observed that wounding the monolayer stimulated a dramatic induction of nuclear rotations at the wound edge, reaching velocities up to 8.5 degrees/min. These nuclear rotations were significantly inhibited in dynein knockdown cells. Surprisingly, centrosomes do not rotate in concert with the nucleus; instead the centrosome remains stably positioned between the nucleus and the leading edge. Together, these results suggest that dynein contributes to migration in two ways: (1) maintaining centrosome centrality by tethering microtubule plus ends at the cortex, and (2) maintaining nuclear centrality by asserting force directly on the nucleus.
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DOI：
10.1083/jcb.118.6.1333
发表时间：
1992-09
期刊：
The Journal of cell biology
影响因子：
--
作者：
Corthésy-Theulaz I;Pauloin A;Pfeffer SR
通讯作者：
Pfeffer SR
影响因子：
7.8
作者：
Lee, Wei-Lih;Oberle, Jessica R;Cooper, John A
通讯作者：
Cooper, John A
DOI：
10.1083/jcb.104.1.87
发表时间：
1987-01
期刊：
The Journal of cell biology
影响因子：
--
作者：
Englander LL;Rubin LL
通讯作者：
Rubin LL
影响因子：
64.5
作者：
Etienne-Manneville, S;Hall, A
通讯作者：
Hall, A
影响因子：
7.8
作者：
Dujardin, Denis L;Barnhart, Lora E;Stehman, Stephanie A;Gomes, Edgar R;Gundersen, Gregg G;Vallee, Richard B
通讯作者：
Vallee, Richard B