The parasitic nematode Oesophagostomum dentatum synthesizes unusual glycosaminoglycan-like O-glycans.
The parasitic nematode Oesophagostomum dentatum synthesizes unusual glycosaminoglycan-like O-glycans.
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DOI：
10.1093/glycob/cwy045
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发表时间：
2018-07-01
期刊：
影响因子：
4.3
通讯作者：
Wilson IB
中科院分区：
文献类型：
--
作者：
Vanbeselaere J;Yan S;Joachim A;Paschinger K;Wilson IB
O-糖基化可能是所有生物体中变化最大的翻译后修饰之一，但也是最难分析的。在动物中，丝氨酸残基的0-木糖基化代表糖胺聚糖合成的第一阶段，糖胺聚糖的重复区域通常作为酶或化学降解产生的片段进行分析，而其核心区域可以通过β-消除或内切-β-木糖苷酶消化分离。在本研究中，我们表明，肼解可以用于释放糖胺聚糖型寡糖从线虫荧光标记之前，2-氨基吡啶。虽然各种[HexNAcHexA] nGal 2Xyl寡糖从模式生物秀丽隐杆线虫中分离出来，但在猪寄生虫齿口线虫中发现了更多不寻常的糖胺聚糖型聚糖。在这种情况下，通过氢氟酸或β-半乳糖苷酶消化前后的MS/MS判断，检测到具有额外半乳糖和磷酸胆碱残基的核心序列为[（±PC）HexNAcHexA]n（±PC）Galβ3-（±Galβ4）Galβ4Xyl。因此，肼解和荧光标记可以结合起来分析独特形式的O-木糖基化，包括两性离子聚糖修饰的新实例。
O-glycosylation is probably one of the most varied sets of post-translational modifications across all organisms, but amongst the most refractory to analyse. In animals, O-xylosylation of serine residues represents the first stage in the synthesis of glycosaminoglycans, whose repeat regions are generally analysed as fragments resulting from enzymatic or chemical degradation, whereas their core regions can be isolated by β-elimination or endo-β-xylosidase digestion. In the present study, we show that hydrazinolysis can be employed for release of glycosaminoglycan-type oligosaccharides from nematodes prior to fluorescent labelling with 2-aminopyridine. While various [HexNAcHexA]nGal2Xyl oligosaccharides were isolated from the model organism Caenorhabditis elegans, more unusual glycosaminoglycan-type glycans were found to be present in the porcine parasite Oesophagostomum dentatum. In this case, as judged by MS/MS before and after hydrofluoric acid or β-galactosidase digestion, core sequences with extra galactose and phosphorylcholine residues were detected as [(±PC)HexNAcHexA]n(±PC)Galβ3-(±Galβ4)Galβ4Xyl. Thus, hydrazinolysis and fluorescent labelling can be combined to analyse unique forms of O-xylosylation, including new examples of zwitterionic glycan modifications.
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影响因子：
4.3
作者：
Paschinger K;Wilson IB
通讯作者：
Wilson IB
无脊椎动物和原生n-聚糖的分析。
DOI：
10.1007/978-1-4939-6493-2_13
发表时间：
2017
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Hykollari, Alba;Paschinger, Katharina;Eckmair, Barbara;Wilson, Iain B H
通讯作者：
Wilson, Iain B H
影响因子：
4.3
作者：
WONGMADDEN, ST;LANDRY, D
通讯作者：
LANDRY, D
影响因子：
2.7
作者：
KON, A;TAKAGAKI, K;ENDO, M
通讯作者：
ENDO, M
影响因子：
2.7
作者：
HASE, S;IBUKI, T;IKENAKA, T
通讯作者：
IKENAKA, T