2D versus 3D human induced pluripotent stem cell-derived cultures for neurodegenerative disease modelling.
2D versus 3D human induced pluripotent stem cell-derived cultures for neurodegenerative disease modelling.
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DOI：
10.1186/s13024-018-0258-4
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发表时间：
2018-05-22
影响因子：
15.1
通讯作者：
Bithell A
中科院分区：
文献类型：
--
作者：
Centeno EGZ;Cimarosti H;Bithell A
神经退行性疾病，例如阿尔茨海默病（AD）、帕金森病（PD）、亨廷顿病（HD）和肌萎缩侧索硬化症（ALS），每年影响数百万人，并且到目前为止，还没有可用的治疗方法。尽管动物和组织学模型在理解疾病机制和确定可能的治疗策略方面有很大的帮助，但为了找到疾病修饰解决方案，仍然迫切需要能够提供更多预测性和生理相关结果的系统。一种可能的途径是开发患者来源的模型，例如通过将患者体细胞重编程为人类诱导多能干细胞（hiPSC），然后可以将其分化为任何细胞类型用于建模。这些系统包含来自供体的关键遗传信息，因此作为研究疾病表型和进展的病理机制以及药物测试平台的工具具有巨大的潜力。hiPSC已在2D系统中广泛培养，但为了模拟人脑的复杂性，3D模型已被提出作为更先进的替代方案。本综述将重点关注使用患者来源的hiPSC来模拟AD、PD、HD和ALS。简而言之，我们将涵盖可用的干细胞，2D和3D培养系统的类型，神经退行性疾病的现有模型，体外模拟这些疾病的障碍，以及该领域的当前前景。
Neurodegenerative diseases, such as Alzheimer’s disease (AD), Parkinson’s disease (PD), Huntington’s disease (HD) and amyotrophic lateral sclerosis (ALS), affect millions of people every year and so far, there are no therapeutic cures available. Even though animal and histological models have been of great aid in understanding disease mechanisms and identifying possible therapeutic strategies, in order to find disease-modifying solutions there is still a critical need for systems that can provide more predictive and physiologically relevant results. One possible avenue is the development of patient-derived models, e.g. by reprogramming patient somatic cells into human induced pluripotent stem cells (hiPSCs), which can then be differentiated into any cell type for modelling. These systems contain key genetic information from the donors, and therefore have enormous potential as tools in the investigation of pathological mechanisms underlying disease phenotype, and progression, as well as in drug testing platforms. hiPSCs have been widely cultured in 2D systems, but in order to mimic human brain complexity, 3D models have been proposed as a more advanced alternative. This review will focus on the use of patient-derived hiPSCs to model AD, PD, HD and ALS. In brief, we will cover the available stem cells, types of 2D and 3D culture systems, existing models for neurodegenerative diseases, obstacles to model these diseases in vitro, and current perspectives in the field.
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DOI：
10.1016/j.mcn.2013.07.007
发表时间：
2013-09
期刊：
Molecular and cellular neurosciences
影响因子：
--
作者：
Burkhardt MF;Martinez FJ;Wright S;Ramos C;Volfson D;Mason M;Garnes J;Dang V;Lievers J;Shoukat-Mumtaz U;Martinez R;Gai H;Blake R;Vaisberg E;Grskovic M;Johnson C;Irion S;Bright J;Cooper B;Nguyen L;Griswold-Prenner I;Javaherian A
通讯作者：
Javaherian A
影响因子：
5.6
作者：
Antoni D;Burckel H;Josset E;Noel G
通讯作者：
Noel G
DOI：
10.1073/pnas.1202922109
发表时间：
2012-04-10
影响因子：
11.1
作者：
Bilican, Bilada;Serio, Andrea;Chandran, Siddharthan
通讯作者：
Chandran, Siddharthan
DOI：
10.1007/978-1-60761-292-6_5
发表时间：
2010-01-01
期刊：
PROTCOLS FOR NEURAL CELL CULTURE, FOURTH EDITION
影响因子：
--
作者：
Blain, Manon;Miron, Veronique E.;Antel, Jack P.
通讯作者：
Antel, Jack P.
影响因子：
64.8
作者：
Birey F;Andersen J;Makinson CD;Islam S;Wei W;Huber N;Fan HC;Metzler KRC;Panagiotakos G;Thom N;O'Rourke NA;Steinmetz LM;Bernstein JA;Hallmayer J;Huguenard JR;Paşca SP
通讯作者：
Paşca SP