Matrix-assisted laser desorption/ionisation mass spectrometry imaging and its development for plant protein imaging.

Matrix-assisted laser desorption/ionisation mass spectrometry imaging and its development for plant protein imaging.
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DOI:
10.1186/1746-4811-7-21
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发表时间:
2011-07-05
期刊:
影响因子:
5.1
通讯作者:
Millar AH
Millar AH
中科院分区:
生物学2区
文献类型:
--
作者:
Grassl J;Taylor NL;Millar AH

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基质辅助激光解吸/电离(MALDI)质谱成像(MSI)使用高质量分辨率飞行时间(ToF)质谱仪的功率,耦合到组织切割表面的激光光栅,以提供对生物组织内生物分子空间分布的新见解。该技术在动物和植物中的历史被认为是蛋白质的分析,这种技术在植物中的潜力进行了讨论。从MALDI-MSI中识别蛋白质生物标志物是一个挑战,并讨论了许多不同的方法来解决这个瓶颈。MALDI-MSI所需的技术考虑进行了审查,这些都是从我们自己的工作和植物样品中的蛋白质的MALDI-MSI协议的例子一起提出。
Matrix-Assisted Laser Desorption/Ionisation (MALDI) mass spectrometry imaging (MSI) uses the power of high mass resolution time of flight (ToF) mass spectrometry coupled to the raster of lasers shots across the cut surface of tissues to provide new insights into the spatial distribution of biomolecules within biological tissues. The history of this technique in animals and plants is considered and the potential for analysis of proteins by this technique in plants is discussed. Protein biomarker identification from MALDI-MSI is a challenge and a number of different approaches to address this bottleneck are discussed. The technical considerations needed for MALDI-MSI are reviewed and these are presented alongside examples from our own work and a protocol for MALDI-MSI of proteins in plant samples.
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