Diversifying selection and host adaptation in two endosymbiont genomes.
Diversifying selection and host adaptation in two endosymbiont genomes.
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DOI：
10.1186/1471-2148-7-68
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发表时间：
2007-04-30
影响因子：
3.4
通讯作者：
McGraw, Elizabeth A.
中科院分区：
文献类型：
--
作者：
Brownlie, Jeremy C.;Adamski, Marcin;Slatko, Barton;McGraw, Elizabeth A.
内共生体Wolbachia pipientis感染广泛的节肢动物和丝虫线虫宿主。这些不同的协会形成了一个有吸引力的模型来理解主机：共生体共同进化。沃尔巴克氏体的普遍性和显著改变宿主生殖生物学的能力也构成了旨在控制昆虫害虫和媒介传播疾病的研究策略的基础。感染线虫的沃尔巴克氏体菌株在遗传学上是不同的，严格垂直传播，并且其宿主需要生长和繁殖。相反，昆虫与沃尔巴克氏体形成了更不稳定的联系。在这些类群中，宿主种群最常见的是感染多态性，水平传播发生在远亲宿主之间，对宿主的直接适合度效应是温和的。尽管多年来对沃尔巴克氏体系统的广泛兴趣，但对介导其与不同宿主的各种相互作用的分子机制知之甚少。我们比较了感染果蝇的沃尔巴克氏体的基因组，wMel和线虫马来丝虫，wBM的外群边缘无形体，以确定基因，经历了多样化的选择沃尔巴克氏体谱系。该研究的目的是确定共生的可能分子机制，并了解不同宿主之间多样性关联的性质。在wMel中选择的流行率远大于wBm。基因有助于DNA代谢，辅因子的生物合成和分泌，积极选择在这两个谱系。在wMel中，更强调DNA修复，细胞分裂，蛋白质稳定性和细胞包膜合成。分泌途径和外表面蛋白编码基因受到选择的高度影响，与宿主：寄生虫理论一致。如果各种辅因子分子的选择证据反映了可能的供应，那么昆虫和线虫沃尔巴克氏体都可能为宿主提供物质。细胞包膜合成，DNA复制和修复机制，热休克，和两个组件开关的选择建议策略昆虫沃尔巴克氏体可能采用科普不同的主机和主机内的环境。
The endosymbiont Wolbachia pipientis infects a broad range of arthropod and filarial nematode hosts. These diverse associations form an attractive model for understanding host:symbiont coevolution. Wolbachia's ubiquity and ability to dramatically alter host reproductive biology also form the foundation of research strategies aimed at controlling insect pests and vector-borne disease. The Wolbachia strains that infect nematodes are phylogenetically distinct, strictly vertically transmitted, and required by their hosts for growth and reproduction. Insects in contrast form more fluid associations with Wolbachia. In these taxa, host populations are most often polymorphic for infection, horizontal transmission occurs between distantly related hosts, and direct fitness effects on hosts are mild. Despite extensive interest in the Wolbachia system for many years, relatively little is known about the molecular mechanisms that mediate its varied interactions with different hosts. We have compared the genomes of the Wolbachia that infect Drosophila melanogaster, wMel and the nematode Brugia malayi, wBm to that of an outgroup Anaplasma marginale to identify genes that have experienced diversifying selection in the Wolbachia lineages. The goal of the study was to identify likely molecular mechanisms of the symbiosis and to understand the nature of the diverse association across different hosts. The prevalence of selection was far greater in wMel than wBm. Genes contributing to DNA metabolism, cofactor biosynthesis, and secretion were positively selected in both lineages. In wMel there was a greater emphasis on DNA repair, cell division, protein stability, and cell envelope synthesis. Secretion pathways and outer surface protein encoding genes are highly affected by selection in keeping with host:parasite theory. If evidence of selection on various cofactor molecules reflects possible provisioning, then both insect as well as nematode Wolbachia may be providing substances to hosts. Selection on cell envelope synthesis, DNA replication and repair machinery, heat shock, and two component switching suggest strategies insect Wolbachia may employ to cope with diverse host and intra-host environments.
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DOI：
10.1016/s1095-6433(03)00014-x
发表时间：
2003-04-01
影响因子：
2.3
作者：
Blair, KL;Geary, TG;Thompson, DP
通讯作者：
Thompson, DP
影响因子：
10.7
作者：
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DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
3.2
作者：
Ansaldi, M;Dubnau, D
通讯作者：
Dubnau, D
影响因子：
2.1
作者：
Fares, MA;Moya, A;Barrio, E
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