MicroRNA-29c Acting on FOS Plays a Significant Role in Nonalcoholic Steatohepatitis Through the Interleukin-17 Signaling Pathway.
MicroRNA-29c Acting on FOS Plays a Significant Role in Nonalcoholic Steatohepatitis Through the Interleukin-17 Signaling Pathway.
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DOI：
10.3389/fphys.2021.597449
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发表时间：
2021
影响因子：
4
通讯作者：
Chen YP
中科院分区：
文献类型：
--
作者：
Cai C;Chen DZ;Tu HX;Chen WK;Ge LC;Fu TT;Tao Y;Ye SS;Li J;Lin Z;Wang XD;Xu LM;Chen YP
非酒精性脂肪肝是西方国家最常见的肝脏疾病，在亚洲国家更为普遍。我们的研究确定动物模型中 TH17/Treg 细胞不平衡。基于我们对非酒精性脂肪肝小鼠 TH17/Treg 细胞失衡机制的兴趣，我们进行了联合生物信息学分析，以进一步研究这一过程。常见的基因测序分析基于一个测序平台的一项试验，其中仅进行基因表达分析和富集分析。我们比较了使用不同测序平台进行的不同试验的不同测序结果，并利用这些分析结果的交集进行联合分析。我们使用生物信息学分析方法进行富集分析和图谱相互作用网络分析并预测潜在的microRNA位点。还设计了动物实验来验证基于定量聚合酶链反应（qPCR）和蛋白质印迹的数据分析结果。我们的结果揭示了 8 个共存的差异表达基因 (DEG) 和 7 个铰链基因。已确定的 DEG 可能通过 IL-17 途径影响非酒精性脂肪变性肝炎。我们发现 microRNA-29c 与 FOS 和 IGFBP1 相互作用。聚合酶链反应分析揭示了 NASH 小鼠中 FOS 和 microRNA-29c 的表达，蛋白质印迹分析表明 FOS 蛋白水平具有相同的趋势。基于这些结果，我们建议 microRNA-29c 通过 IL-17 信号通路作用于 FOS，从而调节 NASH 患者的 TH17/Treg 细胞。
Nonalcoholic fatty liver disease is the most common hepatic disease in western countries and is even more ubiquitous in Asian countries. Our study determined that TH17/Treg cells were imbalanced in animal models. Based on our interest in the mechanism underlying TH17/Treg cell imbalance in nonalcoholic fatty liver mice, we conducted a joint bioinformatics analysis to further investigate this process. Common gene sequencing analysis was based on one trial from one sequencing platform, where gene expression analysis and enrichment analysis were the only analyses performed. We compared different sequencing results from different trials performed using different sequencing platforms, and we utilized the intersection of these analytical results to perform joint analysis. We used a bioinformatics analysis method to perform enrichment analysis and map interaction network analysis and predict potential microRNA sites. Animal experiments were also designed to validate the results of the data analysis based on quantitative polymerase chain reaction (qPCR) and western blotting. Our results revealed 8 coexisting differentially expressed genes (DEGs) and 7 hinge genes. The identified DEGs may influence nonalcoholic steatosis hepatitis through the interleukin-17 pathway. We found that microRNA-29c interacts with FOS and IGFBP1. Polymerase chain reaction analyses revealed both FOS and microRNA-29c expression in NASH mice, and western blot analyses indicated the same trend with regard to FOS protein levels. Based on these results, we suggest that microRNA-29c acts on FOS via the interleukin-17 signaling pathway to regulate TH17/Treg cells in NASH patients.
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影响因子：
13.5
作者：
Harley, Isaac T. W.;Stankiewicz, Traci E.;Giles, Daniel A.;Softic, Samir;Flick, Leah M.;Cappelletti, Monica;Sheridan, Rachel;Xanthakos, Stavra A.;Steinbrecher, Kris A.;Sartor, R. Balfour;Kohli, Rohit;Karp, Christopher L.;Divanovic, Senad
通讯作者：
Divanovic, Senad
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10.1038/mtna.2015.23
发表时间：
2015-09-15
期刊：
Molecular therapy. Nucleic acids
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作者：
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作者：
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作者：
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