Malaria. A forward genetic screen identifies erythrocyte CD55 as essential for Plasmodium falciparum invasion.
Malaria. A forward genetic screen identifies erythrocyte CD55 as essential for Plasmodium falciparum invasion.
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DOI：
10.1126/science.aaa3526
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发表时间：
2015-05-08
期刊：
影响因子：
--
通讯作者：
Duraisingh MT
中科院分区：
文献类型：
--
作者：
Egan ES;Jiang RH;Moechtar MA;Barteneva NS;Weekes MP;Nobre LV;Gygi SP;Paulo JA;Frantzreb C;Tani Y;Takahashi J;Watanabe S;Goldberg J;Paul AS;Brugnara C;Root DE;Wiegand RC;Doench JG;Duraisingh MT
确定疟疾宿主决定因素的努力受到阻碍，因为红细胞中没有细胞核，无法在寄生虫复制的细胞中进行遗传操作。我们使用来自造血干细胞的培养红细胞进行恶性疟原虫宿主决定簇的正向遗传筛选。我们发现CD 55是恶性疟原虫入侵的重要宿主因子。CD 55-null红细胞对所有恶性疟原虫分离株的侵袭都是难治的，因为寄生虫未能正确地附着在红细胞表面。因此，CD 55是开发疟疾治疗剂的有吸引力的靶标。基于造血干细胞的正向遗传筛选可能对鉴定疟疾发病机制的其他宿主决定因素有价值。
Efforts to identify host determinants for malaria have been hindered by the absence of a nucleus in erythrocytes, precluding genetic manipulation in the cell where the parasite replicates. We used cultured red blood cells derived from hematopoietic stem cells to carry out a forward genetic screen for Plasmodium falciparum host determinants. We found that CD55 is an essential host factor for P. falciparum invasion. CD55-null erythrocytes were refractory to invasion by all isolates of P. falciparum because parasites failed to attach properly to the erythrocyte surface. Thus, CD55 is an attractive target for the development of malaria therapeutics. Hematopoietic stem cell-based forward genetic screens may be valuable for the identification of additional host determinants of malaria pathogenesis.
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来自1,092个人基因组的遗传变异的综合图。
影响因子：
64.8
作者：
通讯作者：
--
影响因子：
30.8
作者：
Jallow, Muminatou;Teo, Yik Ying;Small, Kerrin S.;Rockett, Kirk A.;Deloukas, Panos;Clark, Taane G.;Kivinen, Katja;Bojang, Kalifa A.;Conway, David J.;Pinder, Margaret;Sirugo, Giorgio;Sisay-Joof, Fatou;Usen, Stanley;Auburn, Sarah;Bumpstead, Suzannah J.;Campino, Susana;Coffey, Alison;Dunham, Andrew;Fry, Andrew E.;Green, Angela;Gwilliam, Rhian;Hunt, Sarah E.;Inouye, Michael;Jeffreys, Anna E.;Mendy, Alieu;Palotie, Aarno;Potter, Simon;Ragoussis, Jiannis;Rogers, Jane;Rowlands, Kate;Somaskantharajah, Elilan;Whittaker, Pamela;Widden, Claire;Donnelly, Peter;Howie, Bryan;Marchini, Jonathan;Morris, Andrew;SanJoaquin, Miguel;Achidi, Eric Akum;Agbenyega, Tsiri;Allen, Angela;Amodu, Olukemi;Corran, Patrick;Djimde, Abdoulaye;Dolo, Amagana;Doumbo, Ogobara K.;Drakeley, Chris;Dunstan, Sarah;Evans, Jennifer;Farrar, Jeremy;Fernando, Deepika;Hien, Tran Tinh;Horstmann, Rolf D.;Ibrahim, Muntaser;Karunaweera, Nadira;Kokwaro, Gilbert;Koram, Kwadwo A.;Lemnge, Martha;Makani, Julie;Marsh, Kevin;Michon, Pascal;Modiano, David;Molyneux, Malcolm E.;Mueller, Ivo;Parker, Michael;Peshu, Norbert;Plowe, Christopher V.;Puijalon, Odile;Reeder, John;Reyburn, Hugh;Riley, Eleanor M.;Sakuntabhai, Anavaj;Singhasivanon, Pratap;Sirima, Sodiomon;Tall, Adama;Taylor, Terrie E.;Thera, Mahamadou;Troye-Blomberg, Marita;Williams, Thomas N.;Wilson, Michael;Kwiatkowski, Dominic P.
通讯作者：
Kwiatkowski, Dominic P.
DOI：
10.1084/jem.149.1.172
发表时间：
1979-01-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Miller LH;Aikawa M;Johnson JG;Shiroishi T
通讯作者：
Shiroishi T
影响因子：
3
作者：
Gwamaka M;Fried M;Domingo G;Duffy PE
通讯作者：
Duffy PE
影响因子：
46.9
作者：
Giarratana, MC;Kobari, L;Douay, L
通讯作者：
Douay, L