Hyperoside promotes pollen tube growth by regulating the depolymerization effect of actin-depolymerizing factor 1 on microfilaments in okra.
Hyperoside promotes pollen tube growth by regulating the depolymerization effect of actin-depolymerizing factor 1 on microfilaments in okra.
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金丝桃苷通过调节肌动蛋白解聚因子1对秋葵微丝的解聚作用促进花粉管生长
DOI：
10.1038/s41438-021-00578-z
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发表时间：
2021-07-01
影响因子：
8.7
通讯作者：
Fu Y
中科院分区：
文献类型：
--
作者：
Dong B;Yang Q;Song Z;Niu L;Cao H;Liu T;Du T;Yang W;Qi M;Chen T;Wang M;Jin H;Meng D;Fu Y
成熟的花粉在柱头上迅速萌发，延伸其花粉管，将精子细胞输送到胚珠进行受精。这一进程的成功是限制产出的一个重要因素。黄酮类化合物在花粉萌发和花粉管生长过程中显著增加，可能在这一过程中起重要作用。然而，这种参与的具体机制还很少被研究。我们前期的研究发现，金丝桃苷能延长黄秋葵的花期，但其具体作用机制尚不清楚。因此，本研究重点研究金丝桃苷对肌动蛋白解聚因子(ADF)的调节作用，进而影响花粉的萌发和生长。结果表明，金丝桃苷可使秋葵的有效授粉期延长2~3倍，并促进花柱内花粉管的生长。然后，我们以烟草细胞为研究系统，发现金丝桃苷加速了细胞间微丝的解聚。金丝桃苷在体外能促进花粉萌发和花粉管伸长。此外，在所有在花粉管中高表达的AeADF基因中，AeADF1被鉴定为对金丝桃苷的响应。此外，体外微丝切断实验表明，金丝桃苷可促进AeADF1介导的微丝消散。在花粉管中，通过寡核苷酸转染法，AeADF1的基因表达水平降低到1/5。AeADF1表达水平的降低部分减弱了金丝桃苷对花粉萌发和花粉管生长的促进作用。本研究为黄酮类化合物在生殖发育中的作用提供了新的研究方向。
Mature pollen germinates rapidly on the stigma, extending its pollen tube to deliver sperm cells to the ovule for fertilization. The success of this process is an important factor that limits output. The flavonoid content increased significantly during pollen germination and pollen tube growth, which suggests it may play an important role in these processes. However, the specific mechanism of this involvement has been little researched. Our previous research found that hyperoside can prolong the flowering period of Abelmoschus esculentus (okra), but its specific mechanism is still unclear. Therefore, in this study, we focused on the effect of hyperoside in regulating the actin-depolymerizing factor (ADF), which further affects the germination and growth of pollen. We found that hyperoside can prolong the effective pollination period of okra by 2–3-fold and promote the growth of pollen tubes in the style. Then, we used Nicotiana benthamiana cells as a research system and found that hyperoside accelerates the depolymerization of intercellular microfilaments. Hyperoside can promote pollen germination and pollen tube elongation in vitro. Moreover, AeADF1 was identified out of all AeADF genes as being highly expressed in pollen tubes in response to hyperoside. In addition, hyperoside promoted AeADF1-mediated microfilament dissipation according to microfilament severing experiments in vitro. In the pollen tube, the gene expression of AeADF1 was reduced to 1/5 by oligonucleotide transfection. The decrease in the expression level of AeADF1 partially reduced the promoting effect of hyperoside on pollen germination and pollen tube growth. This research provides new research directions for flavonoids in reproductive development.
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影响因子：
5.4
作者：
Cao, Jianwen;Tang, Cheng;Yan, Bing Chun
通讯作者：
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影响因子：
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作者：
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