Three cotton genes preferentially expressed in flower tissues encode actin-depolymerizing factors which are involved in F-actin dynamics in cells.
Three cotton genes preferentially expressed in flower tissues encode actin-depolymerizing factors which are involved in F-actin dynamics in cells.
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在花组织中优先表达的三个棉花基因编码参与细胞中 F-肌动蛋白动力学的肌动蛋白解聚因子
DOI：
10.1093/jxb/erp280
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发表时间：
2010
影响因子：
6.9
通讯作者：
Xu WL
中科院分区：
文献类型：
--
作者：
Li XB;Xu D;Wang XL;Huang GQ;Luo J;Li DD;Zhang ZT;Xu WL
为了研究肌动蛋白解聚因子基因的高表达是否与花粉发育相关，在棉花中分离并鉴定了三个 GhADF 基因 (cDNA)。其中，GhADF6和GhADF8优先在花瓣中表达，而GhADF7在花药中表达量最高，揭示了其花药特异性。花药中的 GhADF7 转录本在开花时达到峰值，表明其表达在花药中受到发育调控。从棉花基因组中分离出包括启动子区域的GhADF7基因。为了证明 GhADF7 启动子的特异性，将包括启动子和 5' 非翻译区在内的 5' 侧翼区域与 GUS 基因融合。组织化学分析表明 GhADF7:GUS 基因在花粉粒中特异性表达。当花粉粒萌发时，在伸长的花粉管中检测到非常强的 GUS 染色。此外，拟南芥中GhADF7基因的过度表达减少了能存活的花粉粒，因此转基因植物部分雄性不育。裂殖酵母（Schizosaccharomyces pombe）中 GhADF7 的过度表达改变了肌动蛋白解聚和聚合的平衡，导致细胞中胞质分裂和多核形成缺陷。综合上述所有结果，推测GhADF7基因可能在花粉发育和萌发中发挥重要作用。
To investigate whether the high expression levels of actin-depolymerizing factor genes are related to pollen development, three GhADF genes (cDNAs) were isolated and characterized in cotton. Among them, GhADF6 and GhADF8 were preferentially expressed in petals, whereas GhADF7 displayed the highest level of expression in anthers, revealing its anther specificity. The GhADF7 transcripts in anthers reached its peak value at flowering, suggesting that its expression is developmentally-regulated in anthers. The GhADF7 gene including the promoter region was isolated from the cotton genome. To demonstrate the specificity of the GhADF7 promoter, the 5′-flanking region, including the promoter and 5′-untranslated region, was fused with the GUS gene. Histochemical assays demonstrated that the GhADF7:GUS gene was specifically expressed in pollen grains. When pollen grains germinated, very strong GUS staining was detected in the elongating pollen tube. Furthermore, overexpression of GhADF7 gene in Arabidopsis thaliana reduced the viable pollen grains and, consequently, transgenic plants were partially male-sterile. Overexpression of GhADF7 in fission yeast (Schizosaccharomyces pombe) altered the balance of actin depolymerization and polymerization, leading to the defective cytokinesis and multinucleate formation in the cells. Given all the above results together, it is proposed that the GhADF7 gene may play an important role in pollen development and germination.
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影响因子：
7
作者：
Dai, Shaojun;Chen, Taotao;Wang, Tai
通讯作者：
Wang, Tai
来自海星卵母细胞的肌动蛋白 - 脱聚合蛋白（Depactin）：特性和与肌动蛋白的相互作用。
影响因子：
7.8
作者：
Mabuchi, I
通讯作者：
Mabuchi, I
影响因子：
4.8
作者：
Gibbon, BC
通讯作者：
Gibbon, BC
影响因子：
11.6
作者：
Dong, CH;Xia, GX;Chua, NH
通讯作者：
Chua, NH
DOI：
10.1083/jcb.105.6.2817
发表时间：
1987-12
期刊：
The Journal of cell biology
影响因子：
--
作者：
Bamburg JR;Bray D
通讯作者：
Bray D