Assessment of Hepatocellular Carcinoma Metastasis Glycobiomarkers Using Advanced Quantitative N-glycoproteome Analysis.
Assessment of Hepatocellular Carcinoma Metastasis Glycobiomarkers Using Advanced Quantitative N-glycoproteome Analysis.
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使用先进的定量 N-糖蛋白质组分析评估肝细胞癌转移糖生物标志物
DOI：
10.3389/fphys.2017.00472
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发表时间：
2017
影响因子：
4
通讯作者：
Liu Y
中科院分区：
文献类型：
--
作者：
Liu T;Shang S;Li W;Qin X;Sun L;Zhang S;Liu Y
Hepatocelluar carcinoma (HCC) is one of the most common malignant tumors with high incidence of metastasis. Glycosylation is involved in fundamental molecular and cell biology process occurring in cancer including metastasis formation. In this study, lectin microarray, lectin blotting, lectin affinity chromatography and tandem 18O stable isotope labeling coupled with liquid chromatography-mass spectrometer (LC-MS) analysis were applied to quantify the changes in N-glycosite occupancy for HCC metastasis serum. Firstly, lectin microarray was used to screen glycoforms and Phaseolus vulgaris Leucoagglutinin (PHA-L) reactive structure (β1,6-GlcNAc branched N-glycan) was found to be increased significantly in HCC patients with metastasis compared with those with non-metastasis. Then, PHA-L affinity glycoproteins were enriched followed by N-glycosite occupancy measurement with strategy of tandem 18O stable isotope labeling. 11 glycoproteins with significantly changed N-glycosite occupancy were identified, they were associated with cell migration, invasion and adhesion through p38 mitogen-activated protein kinase signaling pathway and nuclear factor kappa B signaling pathway. Quantification of N-glycosite occupancy for PHA-L reactive glycoproteins could help to discover important glycoproteins of potential clinically significance in terms of HCC etiology. Also, understanding of N-glycosite occupancy alterations will aid the characterization of molecular mechanism of HCC metastasis as well as establishment of novel glycobiomarkers.
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影响因子：
6.3
作者：
Kumada, Takashi;Toyoda, Hidenori;Tada, Toshifumi;Kiriyama, Seiki;Tanikawa, Makoto;Hisanaga, Yasuhiro;Kanamori, Akira;Tanaka, Junko;Kagebayashi, Chiaki;Satomura, Shinji
通讯作者：
Satomura, Shinji
影响因子：
3.8
作者：
Alsenaidy MA;Okbazghi SZ;Kim JH;Joshi SB;Middaugh CR;Tolbert TJ;Volkin DB
通讯作者：
Volkin DB
通过亲和富集和质谱鉴定对肝细胞癌细胞进行表面糖蛋白组学分析
影响因子：
3
作者：
Mi, Wei;Jia, Wei;Qian, Xiaohong
通讯作者：
Qian, Xiaohong
影响因子：
5.2
作者：
Huang, Binbin;Wu, Qiong;Wang, Zhirong
通讯作者：
Wang, Zhirong
影响因子：
9.3
作者：
Kuzmanov U;Kosanam H;Diamandis EP
通讯作者：
Diamandis EP