Effect of phenolic acids of microbial origin on production of reactive oxygen species in mitochondria and neutrophils.
Effect of phenolic acids of microbial origin on production of reactive oxygen species in mitochondria and neutrophils.
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DOI：
10.1186/1423-0127-19-89
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发表时间：
2012-10-12
影响因子：
11
通讯作者：
Fedotcheva N
中科院分区：
文献类型：
--
作者：
Beloborodova N;Bairamov I;Olenin A;Shubina V;Teplova V;Fedotcheva N
几种低分子量的酚酸在脓毒症患者的血液中含量很高。大多数酚酸在人体内的微生物来源已被证实，但其病理生理作用尚不清楚。脓毒症与循环和受累器官中活性氧（ROS）的过量产生有关。在这项工作中，研究了酚酸对线粒体和中性粒细胞ROS产生的影响。线粒体和中性粒细胞中ROS的产生是通过MCLA和luminol依赖的化学发光来测定的。用极谱法测定线粒体耗氧量。使用TPP+选择性电极记录线粒体内膜电位的差异。采用气相色谱-质谱联用方法研究了人体厌氧菌群和好氧致病菌主要类群成员在单培养物中酚类代谢物的形成。所有酚酸均对线粒体和中性粒细胞有影响，它们是组织和循环中ROS的主要产生者。对线粒体产生促氧化作用的酚酸（苯甲酸和肉桂酸）抑制中性粒细胞中ROS的形成。它们对线粒体的影响被二硫苏糖醇（DTT）所消除。苯基乳酸和对羟基苯基乳酸均能降低线粒体和中性粒细胞中ROS的产生。双歧杆菌和乳酸杆菌在体外产生大量的苯乳酸和对羟基苯乳酸，梭状芽胞杆菌在体外产生大量的苯丙酸和对羟基苯丙酸，铜绿假单胞菌和鲍曼不动杆菌在体外产生对羟基苯乙酸；和苯甲酸，由粘质沙雷氏菌产生。线粒体中ROS生成最有效的激活剂是酚酸，其作用是通过与巯基的相互作用介导的。其中有苯甲酸和肉桂酸。一些酚酸，特别是苯乳酸和对羟基苯乳酸，可以减少线粒体和中性粒细胞中ROS的产生，可以发挥天然抗氧化剂的作用。结果表明，微生物来源的低分子量酚酸参与循环和组织中ROS产生的调节，从而影响脓毒症的氧化应激水平。
Several low-molecular-weight phenolic acids are present in the blood of septic patients at high levels. The microbial origin of the most of phenolic acids in the human body was shown previously, but pathophysiological role of the phenolic acids is not clear. Sepsis is associated with the excessive production of reactive oxygen species (ROS) in both the circulation and the affected organs. In this work the influence of phenolic acids on ROS production in mitochondria and neutrophils was investigated. ROS production in mitochondria and neutrophils was determined by MCLA- and luminol-dependent chemiluminescence. The rate of oxygen consumption by mitochondria was determined polarographically. The difference of electric potentials on the inner mitochondrial membrane was registered using a TPP+-selective electrode. The formation of phenolic metabolites in monocultures by the members of the main groups of the anaerobic human microflora and aerobic pathogenic bacteria was investigated by the method of gas chromatography–mass spectrometry. All phenolic acids had impact on mitochondria and neutrophils, the main producers of ROS in tissues and circulation. Phenolic acids (benzoic and cinnamic acids) producing the pro-oxidant effect on mitochondria inhibited ROS formation in neutrophils. Their effect on mitochondria was abolished by dithiothreitol (DTT). Phenyllactate and p-hydroxyphenyllactate decreased ROS production in both mitochondria and neutrophils. Bifidobacteria and lactobacilli produced in vitro considerable amounts of phenyllactic and p-hydroxyphenyllactic acids, Clostridia s. produced great quantities of phenylpropionic and p-hydroxyphenylpropionic acids, p-hydroxyphenylacetic acid was produced by Pseudomonas aeruginosa and Acinetobacter baumanii; and benzoic acid, by Serratia marcescens. The most potent activators of ROS production in mitochondria are phenolic acids whose effect is mediated via the interaction with thiol groups. Among these are benzoic and cinnamic acids. Some phenolic acids, in particular phenyllactate and p-hydroxyphenyllactate, which decrease ROS production in mitochondria and neutrophils, can play a role of natural antioxidants. The results indicate that low-molecular weight phenolic acids of microbial origin participate in the regulation of the ROS production in both the circulation and tissues, thereby affecting the level of oxidative stress in sepsis.
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影响因子：
4.2
作者：
Déprez, S;Brezillon, C;Scalbert, A
通讯作者：
Scalbert, A
DOI：
10.1152/ajpregu.00432.2003
发表时间：
2004-03-01
影响因子：
2.8
作者：
Brealey, D;Karyampudi, S;Singer, M
通讯作者：
Singer, M
DOI：
10.1002/bio.1170100306
发表时间：
1995-05-01
期刊：
JOURNAL OF BIOLUMINESCENCE AND CHEMILUMINESCENCE
影响因子：
--
作者：
DIAZ, AN;SANCHEZ, FG;GARCIA, JAG
通讯作者：
GARCIA, JAG
影响因子：
3.7
作者：
Kumar, Sachin;Patel, Satyananda;Dikshit, Madhu
通讯作者：
Dikshit, Madhu
影响因子：
4.2
作者：
Scalbert, A;Williamson, G
通讯作者：
Williamson, G