Interrelationships between Fatty Acid Composition, Staphyloxanthin Content, Fluidity, and Carbon Flow in the Staphylococcus aureus Membrane.
Interrelationships between Fatty Acid Composition, Staphyloxanthin Content, Fluidity, and Carbon Flow in the Staphylococcus aureus Membrane.
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DOI：
10.3390/molecules23051201
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发表时间：
2018-05-17
期刊：
影响因子：
--
通讯作者：
Wilkinson BJ
中科院分区：
文献类型：
--
作者：
Tiwari KB;Gatto C;Wilkinson BJ
脂肪酸在决定膜的生物物理特性中起着重要的作用。金黄色葡萄球菌产生支链脂肪酸（BCFAs）和直链饱和脂肪酸（scsfa），并可直接结合外源的scsfa和直链不饱和脂肪酸（SCUFAs）。许多金黄色葡萄球菌菌株产生三萜色素葡萄黄质，BCFAs、scsfa和葡萄黄质的平衡决定了膜的流动性。在这里，我们研究了金黄色葡萄球菌中脂肪酸和类胡萝卜素生产的关系，使用了色素菌株（Pig1），其类胡萝卜素缺乏突变体（Pig1ΔcrtM）和天然无色素的阿根廷葡萄球菌，缺乏类胡萝卜素生物合成基因，与金黄色葡萄球菌密切相关。在一定的培养条件下，所有菌株的脂肪酸组成相似，表明葡萄黄质不影响脂肪酸组成。通过荧光各向异性测量，与其他菌株相比，菌株Pig1在所有条件下都具有降低的膜流动性，这表明葡萄黄质有助于维持膜刚性。我们没有发现crtM与脂肪酸生物合成基因表达相关的证据。添加葡萄糖的培养基增加了SCSFA的产量，减少了BCFA和葡萄黄质的产量，而添加乙酸也减少了BCFA，但增加了葡萄黄质的产量。我们认为葡萄黄质水平更多地受到代谢调节的影响，而不是对纳入膜的脂肪酸的反应。
Fatty acids play a major role in determining membrane biophysical properties. Staphylococcus aureus produces branched-chain fatty acids (BCFAs) and straight-chain saturated fatty acids (SCSFAs), and can directly incorporate exogenous SCSFAs and straight-chain unsaturated fatty acids (SCUFAs). Many S. aureus strains produce the triterpenoid pigment staphyloxanthin, and the balance of BCFAs, SCSFAs and staphyloxanthin determines membrane fluidity. Here, we investigated the relationship of fatty acid and carotenoid production in S. aureus using a pigmented strain (Pig1), its carotenoid-deficient mutant (Pig1ΔcrtM) and the naturally non-pigmented Staphylococcus argenteus that lacks carotenoid biosynthesis genes and is closely related to S. aureus. Fatty acid compositions in all strains were similar under a given culture condition indicating that staphyloxanthin does not influence fatty acid composition. Strain Pig1 had decreased membrane fluidity as measured by fluorescence anisotropy compared to the other strains under all conditions indicating that staphyloxanthin helps maintain membrane rigidity. We could find no evidence for correlation of expression of crtM and fatty acid biosynthesis genes. Supplementation of medium with glucose increased SCSFA production and decreased BCFA and staphyloxanthin production, whereas acetate-supplementation also decreased BCFAs but increased staphyloxanthin production. We believe that staphyloxanthin levels are influenced more through metabolic regulation than responding to fatty acids incorporated into the membrane.
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DOI：
10.1099/00221287-6-1-2-95
发表时间：
1952-01-01
期刊：
JOURNAL OF GENERAL MICROBIOLOGY
影响因子：
--
作者：
DUTHIE, ES;LORENZ, LL
通讯作者：
LORENZ, LL
影响因子：
3.1
作者：
Hall, Jeffrey W.;Yang, Junshu;Ji, Yinduo
通讯作者：
Ji, Yinduo
SsrA (tmRNA) 作为反义 RNA，通过与 crtMN mRNA 碱基配对来调节金黄色葡萄球菌色素合成
影响因子：
3.5
作者：
Liu, Yu;Wu, Na;Yang, Guang
通讯作者：
Yang, Guang
影响因子：
6.4
作者：
Fey PD;Endres JL;Yajjala VK;Widhelm TJ;Boissy RJ;Bose JL;Bayles KW
通讯作者：
Bayles KW
影响因子：
3.2
作者：
Kullik, I;Giachino, P;Fuchs, T
通讯作者：
Fuchs, T