Targeted lipidomics and transcriptomics profiling reveal the heterogeneity of visceral and subcutaneous white adipose tissue.
Targeted lipidomics and transcriptomics profiling reveal the heterogeneity of visceral and subcutaneous white adipose tissue.
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靶向脂质组学和转录组学分析揭示内脏和皮下白色脂肪组织的异质性
DOI：
10.1016/j.lfs.2020.117352
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发表时间：
2020-03-15
期刊：
影响因子：
6.1
通讯作者：
Du G
中科院分区：
文献类型：
--
作者：
Hou B;Zhao Y;He P;Xu C;Ma P;Lam SM;Li B;Gil V;Shui G;Qiang G;Liew CW;Du G
内脏脂肪组织（VAT）和皮下脂肪组织（SAT）脂质组的储库特异性差异反映了白色脂肪组织（WAT）的异质性，白色脂肪组织在其对外界刺激的独特反应中发挥着核心作用。然而，储库特异性 WAT 的详细脂质组在很大程度上是未知的，尤其是包括磷脂和鞘脂在内的次要成分。为了研究这一领域，我们在雄性 C57BL/6J 小鼠中应用了 VAT 和 SAT 的高覆盖率靶向脂质组学方法来比较其脂质谱的基础水平。总共鉴定出来自 19 个脂质类别的 342 种脂质。我们的结果表明，TAG 和 FFA 的组成在链长和饱和度方面不同。有趣的是，低丰度磷脂、鞘脂和心磷脂在 SAT 中显着较高。脂质相关网络分析证明TAG和磷脂形成不同的子网，并且与其他脂质种类有更多的联系。对LIPID MAPS和微阵列筛选的基因进行丰富本体分析表明差异主要涉及脂质代谢、胰岛素抵抗和炎症反应。我们全面的脂质组学和转录组学分析揭示了两种特定仓库的 WAT 的脂质组成和脂质代谢差异，这将为内脏和皮下白色脂肪组织异质性的研究提供新的见解。
The depot-specific differences in lipidome of visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT) reflect heterogeneity of white adipose tissue (WAT), which plays a central role in its distinct response to outside stimuli. However, the detailed lipidome of depot-specific WAT is largely unknown, especially the minor constitutes including phospholipid and sphingolipid. To investigate this field, we applied a high-coverage targeted lipidomics approach of VAT and SAT in male C57BL/6J mice to compare the basal level of their lipid profiles. In total, 342 lipid species from 19 lipid classes were identified. Our results showed the composition of TAG and FFA was different in length of chain and saturation. Interestingly, low abundance phospholipid, sphingolipid and cardiolipin were significantly higher in SAT. Lipid correlation network analysis vindicated that TAG and phospholipid formed distinct subnet and had more connections with other lipid species. Enriched ontology analysis of gene screened from LIPID MAPS and microarray suggested the differences were mainly involved in lipid metabolism, insulin resistance and inflammatory response. Our comprehensive lipidomics and transcriptomics analyses revealed differences in lipid composition and lipid metabolism of two depot-specific WAT, which would offer new insights into the investigation of heterogeneity of visceral and subcutaneous white adipose tissue.
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影响因子：
4.2
作者：
Lam, Sin Man;Wang, Yuting;Shui, Guanghou
通讯作者：
Shui, Guanghou
影响因子：
3.7
作者：
Caesar R;Manieri M;Kelder T;Boekschoten M;Evelo C;Müller M;Kooistra T;Cinti S;Kleemann R;Drevon CA
通讯作者：
Drevon CA
影响因子：
6
作者：
Afshinnia F;Rajendiran TM;Karnovsky A;Soni T;Wang X;Xie D;Yang W;Shafi T;Weir MR;He J;Brecklin CS;Rhee EP;Schelling JR;Ojo A;Feldman H;Michailidis G;Pennathur S
通讯作者：
Pennathur S
影响因子：
4.8
作者：
Chan, Robin B.;Oliveira, Tiago G.;Di Paolo, Gilbert
通讯作者：
Di Paolo, Gilbert
DOI：
10.1073/pnas.0601752103
发表时间：
2006-04-25
影响因子：
11.1
作者：
Gesta, S;Blüher, M;Kahn, CR
通讯作者：
Kahn, CR