Biological and immunological characterization of influenza virus haemagglutinin expressed from the haemagglutinin locus of vaccinia virus.
Biological and immunological characterization of influenza virus haemagglutinin expressed from the haemagglutinin locus of vaccinia virus.
复制标题
痘苗病毒血凝素基因座表达的流感病毒血凝素的生物学和免疫学特征。
作者：
S. Itamura;Y. Morikawa;H. Shida;K. Nerome;A. Oya
构建质粒载体，促进外源基因在牛痘病毒血凝素（HA）基因位点的插入和表达。邻近痘苗病毒P7.5启动子的五个独特克隆位点允许将编码蛋白质的外源序列快速插入这些质粒中。该载体系统提供了一个简单的程序来选择重组病毒，因为它们可以很容易地根据其ha缺陷表型进行鉴定。构建了表达流感病毒HA的重组痘苗病毒，以表征该系统的可能用途。重组病毒通过真实的生物合成途径表达流感HA。除了具有与真实流感HA相似的免疫学特性外，表达的HA还具有血凝、血吸附和酸诱导融合活性。这些发现证明了该真核载体系统的实用性。
Plasmid vectors were constructed to facilitate the insertion and expression of a foreign gene in the haemagglutinin (HA) gene locus of vaccinia virus. Five unique cloning sites adjacent to the P7.5 promoter of vaccinia virus permit the rapid insertion of a foreign sequence coding for a protein into these plasmids. This vector system provides a simple procedure to select recombinant viruses because they can be readily identified on the basis of their HA-defective phenotype. Recombinant vaccinia viruses expressing influenza virus HA were constructed to characterize the possible use of this system. The recombinant viruses did express the influenza HA through the authentic pathway of biosynthesis. In addition to having immunological characteristics similar to the authentic influenza HA, the expressed HA was found to possess haemagglutinating, haemadsorption and acid-inducible fusion activities. These findings demonstrate the usefulness of this eukaryotic vector system.
DOI：
10.1073/pnas.82.6.1785
发表时间：
1985-01-01
影响因子：
11.1
作者：
YEWDELL, JW;BENNINK, JR;MOSS, B
通讯作者：
MOSS, B
病毒糖蛋白的表面表达在重组痘苗病毒载体感染的上皮细胞中呈极化。
DOI：
10.1002/j.1460-2075.1986.tb04204.x
发表时间：
1986
期刊：
The EMBO journal
影响因子：
--
作者：
Stephens,EB;Compans,RW;Earl,P;Moss,B
通讯作者：
Moss,B
将未选择的标记有效定向插入痘苗病毒基因组中。
影响因子：
3.7
作者：
Fathi,Z;Sridhar,P;Pacha,RF;Condit,RC
通讯作者：
Condit,RC