Identification and developmental expression of the enzymes responsible for dopamine, histamine, octopamine and serotonin biosynthesis in the copepod crustacean Calanus finmarchicus.
Identification and developmental expression of the enzymes responsible for dopamine, histamine, octopamine and serotonin biosynthesis in the copepod crustacean Calanus finmarchicus.
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DOI：
10.1016/j.ygcen.2013.10.003
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发表时间：
2014-01-01
影响因子：
2.7
通讯作者：
Lenz, Petra H.
中科院分区：
文献类型：
--
作者：
Christie, Andrew E.;Fontanilla, Tiana M.;Roncalli, Vittoria;Cieslak, Matthew C.;Lenz, Petra H.
关键词：
神经化学物质可能在桡足类甲壳类动物Calanus finmarchicus的生理/行为控制中发挥关键作用，Calanus finmarchicus是北大西洋大部分生物量占主导地位的浮游动物。先前，一个由206,041个独特序列组成的从头组装转录组被用来表征Calanus的肽能信号系统。在这里，这个组装被挖掘为编码胺生物合成酶的转录本。以已知的黑腹果蝇蛋白为模板，转录本编码推测的Calanus同源物：色氨酸-苯丙氨酸羟化酶（多巴胺、章鱼胺和血清素的生物合成）、酪氨酸羟化酶（多巴胺和血清素的生物合成）、多巴脱羧酶（多巴胺和血清素的生物合成）、组氨酸脱羧酶（组胺的生物合成）、酪氨酸脱羧酶（章鱼胺的生物合成）。鉴定了酪胺β-羟化酶（章鱼胺生物合成）和色氨酸羟化酶（血清素生物合成）。反向BLAST和结构域分析表明，从这些转录本中推断出的蛋白质具有序列同源性和各自酶家族的结构特征。发育分析显示，所有转录本的表达模式非常一致，在早期鹦鹉螺和早期桡足动物中表达水平最高。这些表达模式提示了胺在发育过程中的作用，特别是在从胚胎到鹦鹉螺和从鹦鹉螺到桡足动物的变质过程中。总的来说，这里提供的数据为未来基于基因的研究奠定了坚实的基础，特别是评估它们在发育控制中可能发挥的作用。
Neurochemicals are likely to play key roles in physiological/behavioral control in the copepod crustacean Calanus finmarchicus, the biomass dominant zooplankton for much of the North Atlantic Ocean. Previously, a de novo assembled transcriptome consisting of 206,041 unique sequences was used to characterize the peptidergic signaling systems of Calanus. Here, this assembly was mined for transcripts encoding enzymes involved in amine biosynthesis. Using known Drosophila melanogaster proteins as templates, transcripts encoding putative Calanus homologs of tryptophan-phenylalanine hydroxylase (dopamine, octopamine and serotonin biosynthesis), tyrosine hydroxylase (dopamine biosynthesis), DOPA decarboxylase (dopamine and serotonin biosynthesis), histidine decarboxylase (histamine biosynthesis), tyrosine decarboxylase (octopamine biosynthesis), tyramine β-hydroxylase (octopamine biosynthesis) and tryptophan hydroxylase (serotonin biosynthesis) were identified. Reverse BLAST and domain analyses show that the proteins deduced from these transcripts possess sequence homology to and the structural hallmarks of their respective enzyme families. Developmental profiling revealed a remarkably consistent pattern of expression for all transcripts, with the highest levels of expression typically seen in the early nauplius and early copepodite. These expression patterns suggest roles for amines during development, particularly in the metamorphic transitions from embryo to nauplius and from nauplius to copepodite. Taken collectively, the data presented here lay a strong foundation for future gene-based studies of aminergic signaling in this and other copepod species, in particular assessment of the roles they may play in developmental control.
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DOI：
10.1016/j.cbd.2012.11.002
发表时间：
2013-03-01
影响因子：
3
作者：
Christie, Andrew E.;Roncalli, Vittoria;Lenz, Petra H.
通讯作者：
Lenz, Petra H.
影响因子：
2.5
作者：
FLAMM, RE;HARRISWARRICK, RM
通讯作者：
HARRISWARRICK, RM
影响因子：
2.5
作者：
FLAMM, RE;HARRISWARRICK, RM
通讯作者：
HARRISWARRICK, RM
影响因子：
2.5
作者：
COURNIL, I;CASASNOVAS, B;BELTZ, BS
通讯作者：
BELTZ, BS
DOI：
10.1073/pnas.231471298
发表时间：
2001-10-23
影响因子：
11.1
作者：
Beltz, BS;Benton, JL;Sullivan, JM
通讯作者：
Sullivan, JM