Soluble ligands for the NKG2D receptor are released during endometriosis and correlate with disease severity.
Soluble ligands for the NKG2D receptor are released during endometriosis and correlate with disease severity.
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DOI：
10.1371/journal.pone.0119961
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Chapron C
中科院分区：
文献类型：
--
作者：
González-Foruria I;Santulli P;Chouzenoux S;Carmona F;Batteux F;Chapron C
子宫内膜异位症是一种良性妇科疾病。大量证据表明，子宫内膜异位症患者存在免疫功能障碍，使异位子宫内膜细胞能够植入和增殖。以往的研究表明，自然杀伤细胞在子宫内膜异位症的免疫控制中起着关键作用。这是一项前瞻性实验室研究，于2011年1月至2013年4月在一家三级护理大学医院进行。我们调查了未怀孕，年龄小于42岁的良性妇科手术患者（n= 202）。在完成手术探查腹部盆腔后，121名组织学证实的子宫内膜异位症妇女和81名无子宫内膜异位症的对照妇女被纳入研究。子宫内膜异位症患者按照手术分类分为浅表性腹膜子宫内膜异位症（SUP）、卵巢子宫内膜异位症（OMA）和深浸润性子宫内膜异位症（DIE）三种不同类型。在手术期间从所有研究参与者获得腹膜液样本，以检测可溶性NKG2D配体（MICA， MICB和ULBP-2）。当排除腹膜液中MICA、MICB和ULBP-2水平检测不到的样本时，子宫内膜异位症患者的MICA比值水平显著高于对照组（中位数为1.1 pg/mg，范围为0.1-143.5，中位数为0.6 pg/mg，范围为0.1-3.5,p=0.003）。子宫内膜异位症患者的腹膜液MICB水平也以类似的方式高于无病女性（中位数为4.6 pg/mg，范围为1.2-4702，中位数为3.4 pg/mg，范围为0.7-20.1,p=0.001）。根据手术分类，与对照组相比，死亡组腹膜液可溶性MICA、MICB和ULBP-2比值水平显著升高（p=0.015、p=0.003和p=0.045）。MICA比值水平与痛经（r=0.232; p=0.029）、总rAFS评分（r=0.221; p=0.031）、粘连rAFS评分（r=0.221; p=0.031）相关。我们证明，在子宫内膜异位症的妇女中，特别是在那些出现死亡的病例中，腹膜液NKG2D配体显著增加。本研究提示NKG2D配体脱落是子宫内膜异位症复杂发病机制中损害NK细胞功能的新途径。
Endometriosis is a benign gynaecological disease. Abundant bulk of evidence suggests that patients with endometriosis have an immunity dysfunction that enables ectopic endometrial cells to implant and proliferate. Previous studies show that natural killer cells have a pivotal role in the immune control of endometriosis. This is a prospective laboratory study conducted in a tertiary-care university hospital between January 2011 and April 2013. We investigated non-pregnant, younger than 42-year-old patients (n= 202) during surgery for benign gynaecological conditions. After complete surgical exploration of the abdominopelvic cavity, 121 women with histologically proven endometriosis and 81 endometriosis-free controls women were enrolled. Patients with endometriosis were classified according to a surgical classification in three different types of endometriosis: superficial peritoneal endometriosis (SUP), ovarian endometrioma (OMA) and deep infiltrating endometriosis (DIE). Peritoneal fluid samples were obtained from all study participants during the surgery in order to detect soluble NKG2D ligands (MICA, MICB and ULBP-2). When samples with undetectable peritoneal fluid levels of MICA, MICB and ULBP-2 were excluded, MICA ratio levels were significantly higher in endometriosis patients than in controls (median, 1.1 pg/mg; range, 0.1–143.5 versus median, 0.6 pg/mg; range, 0.1–3.5; p=0.003). In a similar manner peritoneal fluid MICB levels were also increased in endometriosis-affected patients compared with disease-free women (median, 4.6 pg/mg; range, 1.2–4702 versus median, 3.4 pg/mg; range, 0.7–20.1; p=0.001). According to the surgical classification, peritoneal fluid soluble MICA, MICB and ULBP-2 ratio levels were significantly increased in DIE as compared to controls (p=0.015, p=0.003 and p=0.045 respectively). MICA ratio levels also correlated with dysmenorrhea (r=0.232; p=0.029), total rAFS score (r=0.221; p=0.031) and adhesions rAFS score (r=0.221; p=0.031). We demonstrate a significant increase of peritoneal fluid NKG2D ligands in women with endometriosis especially in those cases presenting DIE. This study suggests that NKG2D ligands shedding is a novel pathway in endometriosis complex pathogenesis that impairs NK cell function.
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影响因子：
6.1
作者：
Chapron, Charles;Chopin, Nicolas;Bricou, Alexandre
通讯作者：
Bricou, Alexandre
影响因子：
56.9
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6.7
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6.7
作者：
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de Ziegler, Dominique
影响因子：
6.7
作者：
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CasanasRoux, F