Local inflammation in fracture hematoma: results from a combined trauma model in pigs.
Local inflammation in fracture hematoma: results from a combined trauma model in pigs.
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DOI：
10.1155/2015/126060
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发表时间：
2015
影响因子：
4.6
通讯作者：
Hildebrand F
中科院分区：
文献类型：
--
作者：
Horst K;Eschbach D;Pfeifer R;Hübenthal S;Sassen M;Steinfeldt T;Wulf H;Ruchholtz S;Pape HC;Hildebrand F
背景。先前的研究表明，在小动物模型中，严重创伤后局部和全身炎症反应之间存在显著的相互作用。本研究的目的是在猪身上建立一种新的联合创伤模型，以研究骨折相关的局部炎症，并获得多处创伤后早期炎症阶段的信息。材料和方法。合并创伤包括胫骨骨折、肺挫伤、肝裂伤和控制性出血。动物机械通气，icu监护48 h。在研究的时间过程中采集血液和骨折血肿样本。采用ELISA试剂盒检测局部和全身血清细胞因子及多种报警因子水平。结果。与假手术组相比，全身血清IL-6和HMGB1值差异有统计学意义。骨折血肿血清中IL-6、IL-8、IL-10、HMGB1与全身炎症反应比较，差异有统计学意义。然而，局部促炎浓度下降，而抗炎介质增加。结论。我们的数据显示了局部和全身炎症反应的时间依赖性激活。事实上，这是第一个在大型动物模型中关注局部和全身炎症反应的研究。
Background. Previous studies showed significant interaction between the local and systemic inflammatory response after severe trauma in small animal models. The purpose of this study was to establish a new combined trauma model in pigs to investigate fracture-associated local inflammation and gain information about the early inflammatory stages after polytrauma. Material and Methods. Combined trauma consisted of tibial fracture, lung contusion, liver laceration, and controlled hemorrhage. Animals were mechanically ventilated and under ICU-monitoring for 48 h. Blood and fracture hematoma samples were collected during the time course of the study. Local and systemic levels of serum cytokines and diverse alarmins were measured by ELISA kit. Results. A statistical significant difference in the systemic serum values of IL-6 and HMGB1 was observed when compared to the sham. Moreover, there was a statistical significant difference in the serum values of the fracture hematoma of IL-6, IL-8, IL-10, and HMGB1 when compared to the systemic inflammatory response. However a decrease of local proinflammatory concentrations was observed while anti-inflammatory mediators increased. Conclusion. Our data showed a time-dependent activation of the local and systemic inflammatory response. Indeed it is the first study focusing on the local and systemic inflammatory response to multiple-trauma in a large animal model.
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影响因子：
--
作者：
De Benedetti, Fabrizio;Rucci, Nadia;Teti, Anna
通讯作者：
Teti, Anna
DOI：
10.1016/j.injury.2012.12.007
发表时间：
2013-08-01
影响因子：
2.5
作者：
Banerjee, Marc;Bouillon, Bertil;Wafaisade, Arasch
通讯作者：
Wafaisade, Arasch
影响因子：
2.9
作者：
Mair KH;Sedlak C;Käser T;Pasternak A;Levast B;Gerner W;Saalmüller A;Summerfield A;Gerdts V;Wilson HL;Meurens F
通讯作者：
Meurens F
影响因子：
3.8
作者：
Currie, Holly N.;Loos, Matthew S.;Boyd, Jonathan W.
通讯作者：
Boyd, Jonathan W.
影响因子：
4.2
作者：
Kolar, Paula;Gaber, Timo;Buttgereit, Frank
通讯作者：
Buttgereit, Frank