Carboxybetaine Modified Interface for Electrochemical Glycoprofiling of Antibodies Isolated from Human Serum.
Carboxybetaine Modified Interface for Electrochemical Glycoprofiling of Antibodies Isolated from Human Serum.
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DOI：
10.1021/acs.langmuir.5b00944
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发表时间：
2015-06-30
期刊：
影响因子：
3.9
通讯作者：
Tkac, Jan
中科院分区：
文献类型：
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作者：
Bertok, Tomas;Sediva, Alena;Filip, Jaroslav;Ilcikova, Marketa;Kasak, Peter;Velic, Dusan;Jane, Eduard;Mravcova, Martina;Rovensky, Jozef;Kunzo, Pavol;Lobotka, Peter;Smatko, Vasilij;Vikartovska, Alica;Tkac, Jan
制备了能够识别与从人血清中分离的抗体相连的聚糖中的两种不同碳水化合物（半乳糖和唾液酸）的阻抗凝集素生物传感器。第一步需要通过自组装单层（SAM）对金表面进行修饰，该自组装单层从含有羧基甜菜碱末端硫醇的溶液中沉积，应用于随后的凝集素共价固定并抵抗非特异性蛋白质吸附。下一步，将黑接骨木凝集素 (SNA) 或蓖麻凝集素 (RCA) 共价连接到 SAM 上，并使用电化学阻抗谱、循环伏安法、石英晶体微天平、接触角测量、zeta 电位测定、X 射线光电子能谱和原子力显微镜等多种技术对构建生物感受层的整个过程进行优化和表征。此外，使用基于 SNA 的凝集素微阵列成功验证了基于 SNA 的凝集素生物传感器在从健康个体和类风湿性关节炎 (RA) 患者的人血清中分离的抗体的糖谱分析中的应用。结果表明，SNA 凝集素能够根据抗体中唾液酸含量的变化区分从健康个体中分离的抗体和从 RA 患者中分离的抗体。此外，应用RCA和SNA生物传感器获得的结果表明，从健康个体中分离的抗体中半乳糖和唾液酸的丰度与年龄相关。
Impedimetric lectin biosensors capable of recognizing two different carbohydrates (galactose and sialic acid) in glycans attached to antibodies isolated from human serum were prepared. The first step entailed the modification of a gold surface by a self-assembled monolayer (SAM) deposited from a solution containing a carboxybetaine-terminated thiol applied to the subsequent covalent immobilization of lectins and to resist nonspecific protein adsorption. In the next step, Sambucus nigra agglutinin (SNA) or Ricinus communis agglutinin (RCA) was covalently attached to the SAM, and the whole process of building a bioreceptive layer was optimized and characterized using a diverse range of techniques including electrochemical impedance spectroscopy, cyclic voltammetry, quartz crystal microbalance, contact angle measurements, zeta-potential assays, X-ray photoelectron spectroscopy, and atomic force microscopy. In addition, the application of the SNA-based lectin biosensor in the glycoprofiling of antibodies isolated from the human sera of healthy individuals and of patients suffering from rheumatoid arthritis (RA) was successfully validated using an SNA-based lectin microarray. The results showed that the SNA lectin, in particular, is capable of discriminating between the antibodies isolated from healthy individuals and those from RA patients based on changes in the amount of sialic acid present in the antibodies. In addition, the results obtained by the application of RCA and SNA biosensors indicate that the abundance of galactose and sialic acid in antibodies isolated from healthy individuals is age-related.
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影响因子：
3.1
作者：
Albrecht, Simone;Unwin, Louise;Rudd, Pauline M.
通讯作者：
Rudd, Pauline M.
影响因子：
3.9
作者：
Abdelsalam, ME;Bartlett, PN;Baumberg, J
通讯作者：
Baumberg, J
影响因子：
16.6
作者：
Dirr, Larissa;El-Deeb, Ibrahim M.;von Itzstein, Mark
通讯作者：
von Itzstein, Mark
影响因子：
6.2
作者：
Klukova L;Bertok T;Petrikova M;Sediva A;Mislovicova D;Katrlik J;Vikartovska A;Filip J;Kasak P;Andicsová-Eckstein A;Mosnáček J;Lukáč J;Rovenský J;Imrich R;Tkac J
通讯作者：
Tkac J
影响因子：
--
作者：
Aletaha, Daniel;Neogi, Tuhina;Hawker, Gillian
通讯作者：
Hawker, Gillian