A pre- and during Pandemic Survey of Sars-Cov-2 Infection in Stray Colony and Shelter Cats from a High Endemic Area of Northern Italy.
A pre- and during Pandemic Survey of Sars-Cov-2 Infection in Stray Colony and Shelter Cats from a High Endemic Area of Northern Italy.
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对意大利北部高流行区流浪猫和收容所猫的 Sars-Cov-2 感染进行大流行前和流行期间的调查。
DOI：
10.3390/v13040618
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发表时间：
2021-04-03
期刊：
影响因子：
--
通讯作者：
Proverbio D
中科院分区：
文献类型：
--
作者：
Spada E;Vitale F;Bruno F;Castelli G;Reale S;Perego R;Baggiani L;Proverbio D
猫容易感染严重急性呼吸综合征冠状病毒2 （SARS-CoV-2）。虽然在世界范围内对养猫进行了许多研究，但关于流浪猫、殖民地猫或收容所猫的数据有限。我们采用血清学和分子方法调查了意大利北部伦巴第地区（SARS-CoV-2高流行区）城市中流浪猫种群在SARS-CoV-2人类暴发前和暴发期间的感染情况。从241只猫身上收集了不同的样本，包括2019年冠状病毒病（COVID-19）大流行前收集的136只猫的冷冻存档血清样本，以及SARS-CoV-2爆发期间收集的105只猫的血清、咽和直肠拭子样本。采用间接酶联免疫吸附试验（ELISA）对所有大流行前样本进行SARS-CoV-2核衣壳抗体血清检测均为阴性，而在大流行期间收集的一份血清样本血清检测呈阳性。SARS-CoV-2抗体与抗猫肠（FECV）和传染性腹膜炎冠状病毒（FIPC）、猫免疫缺陷病毒（FIV）、猫杯状病毒（FCV）、猫疱疹病毒-1 （FHV-1）、猫细小病毒（FPV）、婴儿利什曼原虫、嗜吞噬细胞无形体、立克次体、刚地弓形虫和猫衣原体抗体无血清学交叉反应。咽拭子和直肠拭子实时逆转录聚合酶链反应（rRT-PCR）未检测出SARS-CoV-2 RNA阳性。我们的数据显示，在COVID-19大流行期间，伦巴第的流浪猫确实感染了SARS-CoV-2，但患病率低于养猫。这应该可以缓解公众对流浪猫成为SARS-CoV-2携带者的担忧。
Cats are susceptible to infection with severe acute respiratory syndrome Coronavirus 2 (SARS-CoV-2). Whilst a number of studies have been performed worldwide on owned cats, limited data are available on stray, colony or shelter cats. We investigated SARS-CoV-2 infection in a stray cat population before and during human outbreaks of SARS-CoV-2 in cities in the Lombardy region in northern Italy, a high endemic region for SARS-CoV-2, using serological and molecular methods. A cohort of different samples were collected from 241 cats, including frozen archived serum samples from 136 cats collected before the 2019 coronavirus disease (COVID-19) pandemic and serum, pharyngeal and rectal swab samples from 105 cats collected during the SARS-CoV-2 outbreak. All pre-pandemic samples tested seronegative for antibodies against the nucleocapsid of SARS-CoV-2 using indirect enzyme linked immunosorbent assay (ELISA) test, while one serum sample collected during the pandemic was seropositive. No serological cross-reactivity was detected between SARS-CoV-2 antibodies and antibodies against feline enteric (FECV) and infectious peritonitis coronavirus (FIPC), Feline Immunodeficiency Virus (FIV), Feline Calicivirus (FCV), Feline Herpesvirus-1 (FHV-1), Feline Parvovirus (FPV), Leishmania infantum, Anaplasma phagocytophilum, Rickettsia spp., Toxoplasma gondii or Chlamydophila felis. No pharyngeal or rectal swab tested positive for SARS-CoV-2 RNA on real time reverse transcription-polymerase chain reaction (rRT-PCR). Our data show that SARS-CoV-2 did infect stray cats in Lombardy during the COVID-19 pandemic, but with lower prevalence than found in owned cats. This should alleviate public concerns about stray cats acting as SARS-CoV-2 carriers.
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影响因子：
4.3
作者：
Sailleau, Corinne;Dumarest, Marine;Le Poder, Sophie
通讯作者：
Le Poder, Sophie
影响因子：
11.8
作者：
Barrs VR;Peiris M;Tam KWS;Law PYT;Brackman CJ;To EMW;Yu VYT;Chu DKW;Perera RAPM;Sit THC
通讯作者：
Sit THC
DOI：
10.3390/pathogens9090746
发表时间：
2020-09-11
期刊：
Pathogens (Basel, Switzerland)
影响因子：
--
作者：
Musso N;Costantino A;La Spina S;Finocchiaro A;Andronico F;Stracquadanio S;Liotta L;Visalli R;Emmanuele G
通讯作者：
Emmanuele G
德国家猫群体中出现 SARS-CoV-2 抗体。
影响因子：
7.8
作者：
Michelitsch A;Hoffmann D;Wernike K;Beer M
通讯作者：
Beer M
影响因子：
33.9
作者：
Newman, Alexandra;Smith, David;Behravesh, Casey Barton
通讯作者：
Behravesh, Casey Barton