Occurrence of Antibodies against SARS-CoV-2 in the Domestic Cat Population of Germany.
Occurrence of Antibodies against SARS-CoV-2 in the Domestic Cat Population of Germany.
复制标题
德国家猫群体中出现 SARS-CoV-2 抗体。
DOI：
10.3390/vaccines8040772
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发表时间：
2020-12-17
期刊：
影响因子：
7.8
通讯作者：
Beer M
中科院分区：
文献类型：
--
作者：
Michelitsch A;Hoffmann D;Wernike K;Beer M
家猫（Felis卡图斯）是受欢迎的伴侣动物，与人类主人密切接触。因此，家猫和人类之间跨物种传播事件的风险始终存在。在严重急性呼吸道综合征冠状病毒2（SARS-CoV-2）出现并在全球迅速传播后不久，家猫在传播周期中的作用受到质疑。在本研究中，首次对德国家猫群体中的抗体发生率进行了大规模调查，以评估自然发生的人猫传播SARS-CoV-2的发病率。于2020年4月至9月收集的920份血清样本，采用间接多菌种ELISA进行筛查。使用间接免疫荧光试验（iIFT）验证阳性样本，并额外检测中和抗体。此外，对血清样本进行猫冠状病毒（FCoV）抗体筛查，以排除所述检测系统中的交叉反应性。总的来说，0.69%（6/920）的血清样本被发现对SARS-CoV-2的抗体阳性，通过ELISA和iIFT。这些反应性血清中的两个也显示中和抗体。未观察到与FCoV特异性抗体的交叉反应性。在德国的家猫群体中发现SARS-CoV-2抗体阳性血清样本，而当时该国的人类感染率仍然相当低，这表明SARS-CoV-2发生了人-猫传播，但没有迹象表明SARS-CoV-2在猫中传播。
Domestic cats (Felis catus) are popular companion animals that live in close contact with their human owners. Therefore, the risk of a trans-species spreading event between domestic cats and humans is ever-present. Shortly after the emergence of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its rapid spread around the world, the role of domestic cats in the transmission cycle was questioned. In the present study, the first large-scale survey of antibody occurrence in the domestic cat population in Germany was conducted, in order to assess the incidence of naturally occurring human to cat transmission of SARS-CoV-2. A total of 920 serum samples, which were collected from April to September of 2020, were screened by an indirect multispecies ELISA. Positive samples were verified using an indirect immunofluorescence test (iIFT) and additionally tested for neutralizing antibodies. Furthermore, serum samples were screened for antibodies against feline coronavirus (FCoV), in order to rule out cross-reactivity in the described test systems. Overall, 0.69% (6/920) of serum samples were found to be positive for antibodies against SARS-CoV-2 by ELISA and iIFT. Two of these reactive sera also displayed neutralizing antibodies. No cross-reactivity with FCoV-specific antibodies was observed. The finding of SARS-CoV-2 antibody-positive serum samples in the domestic cat population of Germany, during a period when the incidence of human infection in the country was still rather low, indicates that human-to-cat transmission of SARS-CoV-2 happens, but there is no indication of SARS-CoV-2 circulation in cats.
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影响因子：
13.2
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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