Comparative Proteomic Analysis Provides Insight into the Key Proteins Involved in Cucumber (Cucumis sativus L.) Adventitious Root Emergence under Waterlogging Stress.
Comparative Proteomic Analysis Provides Insight into the Key Proteins Involved in Cucumber (Cucumis sativus L.) Adventitious Root Emergence under Waterlogging Stress.
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比较蛋白质组学分析提供了对参与黄瓜 (Cucumis sativus L.) 涝胁迫下不定根萌发的关键蛋白质的见解
DOI：
10.3389/fpls.2016.01515
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发表时间：
2016
影响因子：
5.6
通讯作者：
Chen X
中科院分区：
文献类型：
--
作者：
Xu X;Ji J;Ma X;Xu Q;Qi X;Chen X
内涝是自然和农业系统中常见的一种非生物胁迫，它主要通过氧气在水中缓慢扩散来影响植物生长。为了在低氧环境中维持根系功能，耐涝植物的一个关键适应是形成不定根（ARs）。我们发现，黄瓜耐涝系Zaoer-N幼苗通过在下胚轴产生大量的AR来适应涝渍胁迫，而敏感系Pepino几乎不产生AR。为了了解AR发生的分子机制，采用基于itraq的定量蛋白质组学方法绘制了Zaoer-N和Pepino在对照和涝渍条件下下胚轴细胞的蛋白质组。共鉴定出5508个蛋白，146个为差异调节蛋白（DRPs），其中耐受性和敏感性蛋白分别为47个和56个。在涝后的Zaoer-N下胚轴中，DRPs与醇脱氢酶（ADH）、1-氨基环丙烷-1-羧酸氧化酶、过氧化物酶、60S核糖体蛋白、GSDL酯酶/脂肪酶、组蛋白去乙酰化酶和组蛋白H5相关，并且在管理能量危机、促进乙烯释放、减少氧化损伤、调动储存脂质和刺激细胞分裂、分化和生长等方面的作用显著过剩。乙烯产量、ADH活性、丙酮酸脱羧酶（PDC）活性和乙醇产量的评价与蛋白质组学结果一致。对相应146个基因的qRT-PCR分析进一步证实了观察到的蛋白丰度的准确性。这些研究结果揭示了渍水诱发黄瓜抗旱性突变的机制，为耐涝黄瓜的选育提供了有价值的信息。
Waterlogging is a common abiotic stress in both natural and agricultural systems, and it primarily affects plant growth by the slow oxygen diffusion in water. To sustain root function in the hypoxic environment, a key adaptation for waterlogging tolerant plants is the formation of adventitious roots (ARs). We found that cucumber waterlogging tolerant line Zaoer-N seedlings adapt to waterlogging stress by developing a larger number of ARs in hypocotyls, while almost no AR is generated in sensitive line Pepino. To understand the molecular mechanisms underlying AR emergence, the iTRAQ-based quantitative proteomics approach was employed to map the proteomes of hypocotyls cells of the Zaoer-N and Pepino under control and waterlogging conditions. A total of 5508 proteins were identified and 146 were differentially regulated proteins (DRPs), of which 47 and 56 DRPs were specific to tolerant and sensitive line, respectively. In the waterlogged Zaoer-N hypocotyls, DRPs related to alcohol dehydrogenases (ADH), 1-aminocyclopropane-1-carboxylicacid oxidases, peroxidases, 60S ribosomal proteins, GSDL esterases/lipases, histone deacetylases, and histone H5 and were strongly overrepresented to manage the energy crisis, promote ethylene release, minimize oxidative damage, mobilize storage lipids, and stimulate cell division, differentiation and growth. The evaluations of ethylene production, ADH activity, pyruvate decarboxylase (PDC) activity and ethanol production were in good agreement with the proteomic results. qRT-PCR analysis of the corresponding 146 genes further confirmed the accuracy of the observed protein abundance. These findings shed light on the mechanisms underlying waterlogging triggered cucumber ARs emergence, and provided valuable information for the breeding of cucumber with enhanced tolerance to waterlogging.
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