Cloning and characterization of the cDNAs for human and rat corticotropin releasing factor-binding proteins
Cloning and characterization of the cDNAs for human and rat corticotropin releasing factor-binding proteins
复制标题
人和大鼠促肾上腺皮质激素释放因子结合蛋白 cDNA 的克隆和表征
作者：
E. Potter;E. Potter;D. Behan;D. Behan;W. Fischer;E. Linton;P. Lowry;W. Vale
促皮质激素释放因子（CRF）1是前阿黑皮素衍生肽的合成和分泌的有效刺激剂。虽然CRF在人体外周循环中的浓度通常较低2 -4，但在整个妊娠期间会增加4 -8，并在分娩后迅速下降。母体血浆CRF可能起源于胎盘，胎盘对生物活性肽5、9、10产生应答，产生肽9及其信使RNA 11。尽管妊娠晚期母体血浆中的CRF浓度与大鼠下丘脑门静脉血中的CRF浓度相似12，13，并且与体外刺激促肾上腺皮质激素（ACTH）释放的CRF浓度相似，但母体血浆ACTH浓度仅随妊娠的推进而略微增加，并保持在正常范围内14。几个研究小组现在已经报道了在人血浆中存在一种CRF结合蛋白，它使CRF 15 -20失活，并被提出用于防止妊娠期不适当的垂体-肾上腺刺激。最近从人血浆中纯化了结合蛋白19。我们现在已经分离并部分测序了结合蛋白，使我们能够从人类肝脏和大鼠大脑中克隆并表征其互补DNA。人和大鼠结合蛋白的cDNA在COS 7细胞中的表达表明，这些蛋白以与天然人蛋白相同的亲和力结合CRF 15。大鼠和人重组结合蛋白均抑制CRF与CRF抗体的结合，并在体外抑制CRF诱导的垂体细胞ACTH释放。
CORTICOTROPIN-releasing factor (CRF)1, is a potent stimulator of synthesis and secretion of preopiomelanocortin-derived peptides. Although CRF concentrations in the human peripheral circulation are normally low2–4, they increase throughout pregnancy4–8 and fall rapidly after parturition. Maternal plasma CRF probably originates from the placenta, which responds to the bioactive peptide5,9,10 and produces the peptide9and its messenger RNA11. Even though CRF concentrations in late gestational maternal plasma are similar to those in rat hypothalamic portal blood12,13 and to those that can stimulate release of adrenocorticotropic hormone (ACTH) in vitro, maternal plasma ACTH concentrations increase only slightly with advancing gestation and remain within the normal range14. Several groups have now reported the existence of a CRF-binding protein in human plasma which inactivates CRF15–20 and which has been proposed to prevent inappropriate pituitary-adrenal stimulation in pregnancy. The binding protein was recently purified from human plasma19. We have now isolated and partially sequenced the binding protein, allowing us to clone and characterize its complementary DNA from human liver and rat brain. Expression of the cDNAs for human and rat binding protein in COS7 cells showed that these proteins bind CRF with the same affinity as the native human protein15. Both rat and human recombinant binding proteins inhibit CRF binding to a CRF antibody and inhibit CRF-induced ACTH release by pituitary cells in vitro.
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DOI：
10.1016/s0021-9258(18)61070-1
发表时间：
1987-07
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
P. Matsudaira
通讯作者：
P. Matsudaira
DOI：
10.1073/pnas.84.20.6970
发表时间：
1987-10-01
影响因子：
11.1
作者：
AEBERSOLD, RH;LEAVITT, J;KENT, SBH
通讯作者：
KENT, SBH
DOI：
10.1210/jcem-55-2-384
发表时间：
1982-08
期刊：
The Journal of clinical endocrinology and metabolism
影响因子：
--
作者：
T. Shibasaki;E. Odagiri;K. Shizume;N. Ling
通讯作者：
T. Shibasaki;E. Odagiri;K. Shizume;N. Ling
影响因子：
5.8
作者：
GOLAND, RS;WARDLAW, SL;FRANTZ, AG
通讯作者：
FRANTZ, AG
药物肾上腺切除术后垂体门静脉血浆水平、中位隆起含量以及促肾上腺皮质激素释放因子、精氨酸加压素和催产素的免疫组织化学染色。
影响因子：
4.8
作者：
Plotsky,PM;Sawchenko,PE
通讯作者：
Sawchenko,PE