Regioselectivity of human UDP-glucuronosyltransferase isozymes in flavonoid biotransformation by metal complexation and tandem mass spectrometry.
Regioselectivity of human UDP-glucuronosyltransferase isozymes in flavonoid biotransformation by metal complexation and tandem mass spectrometry.
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人UDP-葡萄糖基转移酶在类黄酮生物转化中通过金属络合和串联质谱法的区域选择性。
DOI：
10.1016/j.bcp.2011.08.015
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发表时间：
2011-12-01
影响因子：
5.8
通讯作者：
Brodbelt JS
中科院分区：
文献类型：
--
作者：
Robotham SA;Brodbelt JS
通过与5种黄酮类化合物的反应，确定了12种人葡萄糖醛基转移酶（UGT）同工酶的区域选择性。根据柱后络合生成的[Co(II)（类黄酮- H）（4,7-二苯基-1,10-菲罗啉）2]+配合物的LC-MS/MS片段模式对不同的类黄酮葡萄糖醛酸酯进行了区分。评估葡萄糖醛酸分布，以便对每个同工酶的区域选择性进行系统评估。各种UGT酶，包括8种UGT1A和4种UGT2B，在葡萄糖醛酸化的位置和与黄酮和黄酮醇的相对反应性方面显示出显著的选择性范围。
Based on reactions with five flavonoids, the regioselectivities of twelve human UDP-glucuronosyltransferase (UGT) isozymes were elucidated. The various flavonoid glucuronides were differentiated based on LC-MS/MS fragmentation patterns of [Co(II)(flavonoid – H)(4,7-diphenyl-1,10-phenanthroline)2]+ complexes generated upon post-column complexation. Glucuronide distributions were evaluated to allow a systematic assessment of the regioselectivity of each isozyme. The various UGT enzymes, including eight UGT1A and four UGT2B, displayed a remarkable range of selectivities, both in terms of the positions of glucuronidation and relative reactivity with flavanones versus flavonols.
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影响因子：
6.1
作者：
Singh, Rashim;Wu, Baojian;Hu, Ming
通讯作者：
Hu, Ming
影响因子：
7.4
作者：
Davis, BD;Brodbelt, JS
通讯作者：
Brodbelt, JS
DOI：
10.1016/j.jasms.2003.07.002
发表时间：
2003-12-01
影响因子：
3.2
作者：
Pikulski, M;Brodbelt, JS
通讯作者：
Brodbelt, JS
LC-ESI-MS-MS 离心超滤法测定金银花主要黄酮类化合物与大鼠血浆蛋白的结合
影响因子：
1.7
作者：
Luo, Yongdong;Wu, Shuisheng;Li, Ping
通讯作者：
Li, Ping
DOI：
10.1016/s1044-0305(01)00236-7
发表时间：
2001-05-01
影响因子：
3.2
作者：
Satterfield, M;Brodbelt, JS
通讯作者：
Brodbelt, JS