Ecological Overlap and Horizontal Gene Transfer in Staphylococcus aureus and Staphylococcus epidermidis.
Ecological Overlap and Horizontal Gene Transfer in Staphylococcus aureus and Staphylococcus epidermidis.
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DOI：
10.1093/gbe/evv066
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发表时间：
2015-04-16
影响因子：
3.3
通讯作者：
Sheppard SK
中科院分区：
文献类型：
--
作者：
Méric G;Miragaia M;de Been M;Yahara K;Pascoe B;Mageiros L;Mikhail J;Harris LG;Wilkinson TS;Rolo J;Lamble S;Bray JE;Jolley KA;Hanage WP;Bowden R;Maiden MC;Mack D;de Lencastre H;Feil EJ;Corander J;Sheppard SK
机会致病菌金黄色葡萄球菌和表皮葡萄球菌是严重医院感染的主要原因，在世界范围内与高死亡率和发病率相关。这些物种在人类皮肤和鼻咽上都是常见的共栖生物，但在基因上是不同的，在1478个核心基因中，核苷酸的平均差异为24%。为了更好地了解这些生态相似的葡萄球菌物种的基因组动力学，我们对324个金黄色葡萄球菌和表皮葡萄球菌基因组进行了比较分析，其中包括83个新的表皮葡萄球菌序列。参考泛基因组方法和全基因组多位点序列分型显示，大约一半的基因组在物种之间共享。基于BratNextGen分析，发现同源重组影响了表皮葡萄球菌40%的核心基因，但仅影响了金黄色葡萄球菌24%的核心基因。物种之间的同源重组是罕见的，在任何两个表皮葡萄球菌和金黄色葡萄球菌分离株之间最多有9个基因等位基因共享。相比之下，有相当多的移动元件在种间混合，特别是与SaPIn1致病性岛、金属解毒和甲氧西林抗性岛SCCmec相关的基因。我们的数据和分析为考虑金黄色葡萄球菌和表皮葡萄球菌之间重组边界的性质以及影响这些物种之间实现重组的选择力提供了一个背景。
The opportunistic pathogens Staphylococcus aureus and Staphylococcus epidermidis represent major causes of severe nosocomial infection, and are associated with high levels of mortality and morbidity worldwide. These species are both common commensals on the human skin and in the nasal pharynx, but are genetically distinct, differing at 24% average nucleotide divergence in 1,478 core genes. To better understand the genome dynamics of these ecologically similar staphylococcal species, we carried out a comparative analysis of 324 S. aureus and S. epidermidis genomes, including 83 novel S. epidermidis sequences. A reference pan-genome approach and whole genome multilocus-sequence typing revealed that around half of the genome was shared between the species. Based on a BratNextGen analysis, homologous recombination was found to have impacted on 40% of the core genes in S. epidermidis, but on only 24% of the core genes in S. aureus. Homologous recombination between the species is rare, with a maximum of nine gene alleles shared between any two S. epidermidis and S. aureus isolates. In contrast, there was considerable interspecies admixture of mobile elements, in particular genes associated with the SaPIn1 pathogenicity island, metal detoxification, and the methicillin-resistance island SCCmec. Our data and analysis provide a context for considering the nature of recombinational boundaries between S. aureus and S. epidermidis and, the selective forces that influence realized recombination between these species.
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核心基因组中最近的重组事件与粪肠球菌的自适应进化有关。
影响因子：
3.3
作者：
de Been M;van Schaik W;Cheng L;Corander J;Willems RJ
通讯作者：
Willems RJ
影响因子：
3.2
作者：
Chan, Cheong Xin;Beiko, Robert G.;Ragan, Mark A.
通讯作者：
Ragan, Mark A.
影响因子：
2.6
作者：
Feil, EJ;Enright, MC;Spratt, BG
通讯作者：
Spratt, BG
影响因子：
3.2
作者：
FORBES, BA;SCHABERG, DR
通讯作者：
SCHABERG, DR
影响因子：
3.2
作者：
Feil, EJ;Li, BC;Spratt, BG
通讯作者：
Spratt, BG