Recent recombination events in the core genome are associated with adaptive evolution in Enterococcus faecium.
Recent recombination events in the core genome are associated with adaptive evolution in Enterococcus faecium.
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核心基因组中最近的重组事件与粪肠球菌的自适应进化有关。
DOI：
10.1093/gbe/evt111
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发表时间：
2013
影响因子：
3.3
通讯作者：
Willems RJ
中科院分区：
文献类型：
--
作者：
de Been M;van Schaik W;Cheng L;Corander J;Willems RJ
粪肠球菌对临床的影响不断上升的原因包括该物种迅速获得适应遗传因素。在这里，我们重点研究重组对粪肠球菌进化的影响。我们使用最近开发的BratNextGen算法检测了34株粪肠球菌核心基因组中的重组区，其中包括3株新测序的临床菌株。重组被发现对粪肠球菌基因组有重大影响：在核心基因组最初的120万个位置中，预计至少有一个菌株的50万个位置受到重组的影响。重要的是，包含大部分人类肠道共生菌的两个主要粪便埃希菌分支之一(分支B)中的菌株，形成了将外来DNA捐赠给第二个主要粪便埃希菌分支(分支A)的最重要的储存库，该分支包含了大多数临床分离株。此外，还发现在特定的医院相关粪肠球菌菌株中，几个基因组区域主要重组。其中一个区域(类似EPA的区域)可能编码细胞壁多糖的生物合成。这些发现表明，重组在粪肠球菌作为一种成功的医院相关病原体的出现中起着至关重要的作用。
Reasons for the rising clinical impact of the bacterium Enterococcus faecium include the species’ rapid acquisition of adaptive genetic elements. Here, we focused on the impact of recombination on the evolution of E. faecium. We used the recently developed BratNextGen algorithm to detect recombinant regions in the core genome of 34 E. faecium strains, including three newly sequenced clinical strains. Recombination was found to have a significant impact on the E. faecium genome: of the original 1.2 million positions in the core genome, 0.5 million were predicted to have been affected by recombination in at least one strain. Importantly, strains in one of the two major E. faecium clades (clade B), which contains most of the E. faecium human gut commensals, formed the most important reservoir for donating foreign DNA to the second major E. faecium clade (clade A), which contains most of the clinical isolates. Also, several genomic regions were found to mainly recombine in specific hospital-associated E. faecium strains. One of these regions (the epa-like locus) likely encodes the biosynthesis of cell wall polysaccharides. These findings suggest a crucial role for recombination in the emergence of E. faecium as a successful hospital-associated pathogen.
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影响因子：
6.7
作者：
Brueggemann, Angela B.;Pai, Rekha;Beall, Bernard
通讯作者：
Beall, Bernard
影响因子：
3.2
作者：
Carias, LL;Rudin, SD;Rice, LB
通讯作者：
Rice, LB
影响因子：
3.2
作者：
Lam, Margaret M. C.;Seemann, Torsten;Stinear, Timothy P.
通讯作者：
Stinear, Timothy P.
影响因子：
6.7
作者：
Leavis HL;Willems RJ;van Wamel WJ;Schuren FH;Caspers MP;Bonten MJ
通讯作者：
Bonten MJ
DOI：
10.1093/bioinformatics/btp348
发表时间：
2009-08-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Capella-Gutiérrez S;Silla-Martínez JM;Gabaldón T
通讯作者：
Gabaldón T