Candida albicans Adhesins Als1 and Hwp1 Modulate Interactions with Streptococcus mutans.
Candida albicans Adhesins Als1 and Hwp1 Modulate Interactions with Streptococcus mutans.
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DOI：
10.3390/microorganisms11061391
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发表时间：
2023-05-25
期刊：
影响因子：
4.5
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中科院分区：
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已知白色念珠菌和变形链球菌在口腔中相互协同作用。例如，变形链球菌分泌的葡萄糖转移酶B(GTFB)可以结合到白色念珠菌的细胞表面，促进双菌种生物膜的形成。然而，介导与变形链球菌相互作用的真菌因素尚不清楚。白念珠菌粘附素Als1、Als3和Hwp1是白念珠菌单种生物膜形成的关键分子，但它们在与变形链球菌相互作用中的作用尚未得到评估。在这里，我们研究了白色念珠菌细胞壁粘附素Als1、Als3和Hwp1在与变形链球菌形成双物种生物膜中的作用。我们通过测量白念珠菌野生型als1Δ/Δ、als3Δ/Δ、als1Δ/Δ/als3Δ/Δ和hwp1Δ/Δ菌株与变形链球菌形成双菌种生物膜的光密度、代谢活性、细胞计数、生物量、厚度和构型来评价它们形成双物种生物膜的能力。我们观察到，在这些不同的生物膜检测中，白色念珠菌野生型菌株在变形链球菌存在的情况下形成了增强的双物种生物膜，证实了白色念珠菌和变形链球菌在生物膜的背景下协同作用。我们的结果表明，白色念珠菌Als1和Hwp1是与变形链球菌相互作用的主要参与者，因为当als1Δ/Δ或hwp1Δ/Δ菌株与变形链球菌在双种生物膜中培养时，不能促进双种生物膜的形成。然而，Als3似乎在与变形链球菌形成双物种生物膜的相互作用中发挥着不明显的作用。总体而言，我们的数据表明，白色念珠菌粘附素Als1和Hwp1具有调节与变形链球菌相互作用的功能，可能成为未来治疗的潜在靶点。
Candida albicans and Streptococcus mutans are known to synergistically interact with each other in the oral cavity. For example, glucosyltransferase B (GtfB), secreted by S. mutans, can bind to the C. albicans cell surface, promoting dual-species biofilm formation. However, the fungal factors mediating interactions with S. mutans are unknown. The C. albicans adhesins Als1, Als3, and Hwp1 are key players in C. albicans single-species biofilm formation, but their roles, if any, in interacting with S. mutans have not been assessed. Here, we investigated the roles of the C. albicans cell wall adhesins Als1, Als3, and Hwp1 on forming dual-species biofilms with S. mutans. We assessed the abilities of the C. albicans wild-type als1Δ/Δ, als3Δ/Δ, als1Δ/Δ/als3Δ/Δ, and hwp1Δ/Δ strains to form dual-species biofilms with S. mutans by measuring optical density, metabolic activity, cell enumeration, biomass, thickness, and architecture of the biofilms. We observed that the C. albicans wild-type strain formed enhanced dual-species biofilms in the presence of S. mutans in these different biofilm assays, confirming that C. albicans and S. mutans synergistically interact in the context of biofilms. Our results reveal that C. albicans Als1 and Hwp1 are major players in interacting with S. mutans, since dual-species biofilm formation was not enhanced when the als1Δ/Δ or hwp1Δ/Δ strains were cultured with S. mutans in dual-species biofilms. Als3, however, does not seem to play a clear role in interacting with S. mutans in dual-species biofilm formation. Overall, our data suggest that the C. albicans adhesins Als1 and Hwp1 function to modulate interactions with S. mutans and could be potential targets for future therapeutics.
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