Expression analysis of genes related to lipid metabolism in peripheral blood lymphocytes of chickens challenged with reticuloendotheliosis virus.
Expression analysis of genes related to lipid metabolism in peripheral blood lymphocytes of chickens challenged with reticuloendotheliosis virus.
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网状内皮增生病毒攻击鸡外周血淋巴细胞脂质代谢相关基因的表达分析
DOI：
10.1016/j.psj.2021.101081
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发表时间：
2021-05
期刊：
影响因子：
4.4
通讯作者：
Chen G
中科院分区：
文献类型：
--
作者：
Bi Y;Yuan X;Chen Y;Chang G;Chen G
禽网状内皮组织增生病病毒（REV）感染鸡外周血淋巴细胞脂质代谢的分子机制尚不清楚。因此，在体外和体内探索了这个科学问题。结果表明，REV感染后血淋巴细胞甘油三酯含量显著降低，游离脂肪酸含量和肉毒碱棕榈酰转移酶-1活性显著升高。通过RNA测序，通过Gene Ontology术语分析筛选出97个已知的与脂代谢或糖代谢相关的差异表达基因。在这些97 DEG的基础上，鉴定了富集的途径，包括过氧化物酶体增殖物激活受体（PPAR）信号传导途径。在这97个DEG中，一些代表性基因与脂解和脂肪酸利用相关（PPARG、LPL、PLIN 2、ACOX 1、ACSL 1、FABP 3和FABP 4）。然而，与脂质生物合成相关的其他基因（ACSL 3、ACSL 6、DGAT 2、LPIN 1和LPIN 2）下调。定量聚合酶链反应结果证实了RNA测序数据的准确性，体内结果支持这些体外结果。结果表明，REV对鸡外周血淋巴细胞脂肪酸和脂质代谢具有调节作用。淋巴细胞被REV感染后，优先使用外源脂肪酸;参与脂肪酸利用的基因通过PPAR途径上调，而参与脂质和脂肪酸生物合成的基因下调。
The underlying molecular mechanism of lipid metabolism in peripheral blood lymphocytes from chicken infected with reticuloendotheliosis virus (REV) remains poorly understood. Therefore, this scientific question was explored in vitro and in vivo. The results indicated that triglyceride content was significantly reduced, but the free fatty acid content and carnitine palmitoyltransferase-1 activity were significantly increased in blood lymphocytes after REV infection. By RNA sequencing, 97 known differentially expressed genes (DEG) related to lipid metabolism or glycometabolism were screened via Gene Ontology term analysis. On the basis of these 97 DEG, enriched pathways, including the peroxisome proliferators-activated receptor (PPAR) signaling pathway, were identified. Among these 97 DEG, some representative genes were related to lipolysis and fatty acid utilization (PPARG, LPL, PLIN2, ACOX1, ACSL1, FABP3, and FABP4). However, other genes related to lipid biosynthesis (ACSL3, ACSL6, DGAT2, LPIN1, and LPIN2) were downregulated. The quantitative polymerase chain reaction results confirmed the accuracy of the RNA sequencing data, and the in vivo outcome supports theses in vitro results. Our findings revealed that REV regulates fatty acid and lipid metabolism in peripheral blood lymphocytes from chicken. After the lymphocytes were infected with REV, the exogenous fatty acids were preferentially used; genes involved in fatty acid utilization were upregulated via the PPAR pathway, whereas genes involved in lipid and fatty acid biosynthesis were downregulated.
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影响因子：
3.4
作者：
Lopes-Marques M;Cunha I;Reis-Henriques MA;Santos MM;Castro LF
通讯作者：
Castro LF
影响因子：
2.9
作者：
Liang M;Mulholland DJ
通讯作者：
Mulholland DJ
网状内皮病病毒抑制了作用于鸡外周血的淋巴细胞上的免疫反应。
影响因子：
4
作者：
Bi Y;Xu L;Qiu L;Wang S;Liu X;Zhang Y;Chen Y;Zhang Y;Xu Q;Chang G;Chen G
通讯作者：
Chen G
DOI：
10.1038/ijo.2017.197
发表时间：
2018-03
期刊：
International journal of obesity (2005)
影响因子：
--
作者：
Jahansouz C;Xu H;Hertzel AV;Kizy S;Steen KA;Foncea R;Serrot FJ;Kvalheim N;Luthra G;Ewing K;Leslie DB;Ikramuddin S;Bernlohr DA
通讯作者：
Bernlohr DA
影响因子：
16.6
作者：
O-Sullivan I;Zhang W;Wasserman DH;Liew CW;Liu J;Paik J;DePinho RA;Stolz DB;Kahn CR;Schwartz MW;Unterman TG
通讯作者：
Unterman TG