Soybean Trihelix transcription factors GmGT-2A and GmGT-2B improve plant tolerance to abiotic stresses in transgenic Arabidopsis.
Soybean Trihelix transcription factors GmGT-2A and GmGT-2B improve plant tolerance to abiotic stresses in transgenic Arabidopsis.
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DOI：
10.1371/journal.pone.0006898
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发表时间：
2009-09-04
期刊：
影响因子：
3.7
通讯作者：
Chen SY
中科院分区：
文献类型：
--
作者：
Xie ZM;Zou HF;Lei G;Wei W;Zhou QY;Niu CF;Liao Y;Tian AG;Ma B;Zhang WK;Zhang JS;Chen SY
三螺旋转录因子在光调控反应和其他发育过程中发挥重要作用。然而，它们在非生物应激反应中的作用在很大程度上尚不清楚。本研究从大豆中鉴定出两个三螺旋转录因子基因GmGT-2A和GmGT-2B，并进一步鉴定了它们在非生物胁迫耐受中的作用。这两个基因都可以被各种非生物胁迫诱导，编码的蛋白都定位于核区。在酵母实验中，GmGT-2B而不是GmGT-2A表现出转录激活和二聚化的能力。GmGT-2B中153个残基的n端肽是最小激活域，两个三螺旋之间的中间区域介导了GmGT-2B的二聚化。在植物细胞中也证实了GmGT-2B的转激活活性。酵母单杂交DNA结合分析表明，GmGT-2A可与GT-1bx、GT-2bx、mGT-2bx-2和D1结合，而GmGT-2B可与后3个元素结合。GmGT-2A和GmGT-2B的过表达提高了转基因拟南芥植株对盐、冷冻和干旱胁迫的耐受性。此外，在ABA处理下，gmgt - 2b转基因植株的绿苗数量比col0多。GmGT-2A-和gmgt - 2b转基因植物中许多应激反应基因发生了改变。这些结果表明，GmGT-2A和GmGT-2B通过调控一组共同基因和一组特定基因来赋予胁迫耐受性。GmGT-2B也影响ABA敏感性。
Trihelix transcription factors play important roles in light-regulated responses and other developmental processes. However, their functions in abiotic stress response are largely unclear. In this study, we identified two trihelix transcription factor genes GmGT-2A and GmGT-2B from soybean and further characterized their roles in abiotic stress tolerance. Both genes can be induced by various abiotic stresses, and the encoded proteins were localized in nuclear region. In yeast assay, GmGT-2B but not GmGT-2A exhibits ability of transcriptional activation and dimerization. The N-terminal peptide of 153 residues in GmGT-2B was the minimal activation domain and the middle region between the two trihelices mediated the dimerization of the GmGT-2B. Transactivation activity of the GmGT-2B was also confirmed in plant cells. DNA binding analysis using yeast one-hybrid assay revealed that GmGT-2A could bind to GT-1bx, GT-2bx, mGT-2bx-2 and D1 whereas GmGT-2B could bind to the latter three elements. Overexpression of the GmGT-2A and GmGT-2B improved plant tolerance to salt, freezing and drought stress in transgenic Arabidopsis plants. Moreover, GmGT-2B-transgenic plants had more green seedlings compared to Col-0 under ABA treatment. Many stress-responsive genes were altered in GmGT-2A- and GmGT-2B-transgenic plants. These results indicate that GmGT-2A and GmGT-2B confer stress tolerance through regulation of a common set of genes and specific sets of genes. GmGT-2B also affects ABA sensitivity.
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影响因子：
5.1
作者：
Buchel, AS;Molenkamp, R;Linthorst, HJM
通讯作者：
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