Long noncoding RNA MAPKAPK5-AS1 promotes colorectal cancer proliferation by partly silencing p21 expression.
Long noncoding RNA MAPKAPK5-AS1 promotes colorectal cancer proliferation by partly silencing p21 expression.
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长非编码RNA MAPKAPK5-AS1通过部分沉默p21表达促进结直肠癌增殖
DOI：
10.1111/cas.13838
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发表时间：
2019-01
期刊：
影响因子：
5.7
通讯作者：
Wang K
中科院分区：
文献类型：
--
作者：
Ji H;Hui B;Wang J;Zhu Y;Tang L;Peng P;Wang T;Wang L;Xu S;Li J;Wang K
结直肠癌(CRC)是世界上第三大常见的恶性肿瘤，而长非编码RNA(LncRNA)在肿瘤的发生中起着重要的作用。在这里，我们报告了一个新的lncRNA，MAPKAPK5-AS1，它在结直肠癌中起着关键的癌基因的作用。此外，我们还试图探讨MAPKAPK5-AS1在体内外肿瘤进展中的作用。采用定量RT-PCR方法检测MAPKAPK5-AS1在结直肠癌组织和细胞中的表达。50例结直肠癌组织中MAPKAPK5-AS1的表达显著上调，且MAPKAPK5-AS1的表达与结直肠癌患者的肿瘤大小和分期有关。MAPKAPK5-AS1基因的敲除可显著抑制大肠癌细胞的增殖并诱导其凋亡。我们还发现p21是MAPKAPK5-AS1的靶点。此外，我们还首次报道了MAPKAPK5-AS1在结直肠癌中的致癌作用。MAPKAPK5-AS1是一种新的判断结直肠癌预后的生物标志物和潜在的候选治疗方案。
Colorectal cancer (CRC) is the third most common malignancy in the world, and long noncoding RNA (lncRNA) plays a critical role in carcinogenesis. Here, we report a novel lncRNA, MAPKAPK5‐AS1, that acts as a critical oncogene in CRC. In addition, we attempted to explore the functions of MAPKAPK5‐AS1 on tumor progression in vitro and in vivo. Quantitative RT‐PCR was used to examine the expression of MAPKAPK5‐AS1 in CRC tissues and cells. Expression of MAPKAPK5‐AS1 was significantly upregulated in 50 CRC tissues, and increased expression of MAPKAPK5‐AS1 was found to be associated with greater tumor size and advanced pathological stage in CRC patients. Knockdown of MAPKAPK5‐AS1 significantly inhibited proliferation and caused apoptosis in CRC cells. We also found that p21 is a target of MAPKAPK5‐AS1. In addition, we are the first to report that MAPKAPK5‐AS1 plays a carcinogenic role in CRC. MAPKAPK5‐AS1 is a novel prognostic biomarker and a potential therapeutic candidate for CRC cancer.
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影响因子：
--
作者：
Bayes, Monica;Heath, Simon;Gut, Ivo Glynne
通讯作者：
Gut, Ivo Glynne
影响因子：
3.5
作者：
Han, Xiufu;Yang, Yanming;Yang, Yiyong
通讯作者：
Yang, Yiyong
影响因子：
5.8
作者：
Munteanu, Aline;Mukherjee, Neelanjan;Ohler, Uwe
通讯作者：
Ohler, Uwe
DOI：
10.2183/pjab.94.017
发表时间：
2018
期刊：
Proceedings of the Japan Academy. Series B, Physical and biological sciences
影响因子：
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作者：
Mino T;Takeuchi O
通讯作者：
Takeuchi O
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DOI：
10.18632/aging.101485
发表时间：
2018-06-24
期刊：
Aging
影响因子：
--
作者：
Dong Q;Wei L;Zhang MQ;Wang X
通讯作者：
Wang X