Physiological and transcriptomic analysis provide novel insight into cobalt stress responses in willow
Physiological and transcriptomic analysis provide novel insight into cobalt stress responses in willow
复制标题
生理学和转录组分析为柳树的钴胁迫反应提供了新的见解
DOI：
10.1038/s41598-020-59177-y
复制
发表时间：
2020-02
影响因子：
4.6
通讯作者：
Hai-Ling Yang
中科院分区：
文献类型：
--
作者：
Yi-Ming Wang;Qi Yang;Hui Xu;Yan-Jing Liu;Hai-Ling Yang
钴（Co）是生物体中几种酶和辅酶的重要组成部分。过量的一氧化碳对植物有很高的毒性。植物对钴胁迫的分子响应机制的认识仍然有限，特别是木本植物。以垂柳（Salix babylonica）杨柳幼苗为材料，采用形态学、生理生化和RNA-seq分析方法，研究了垂柳幼苗对Co胁迫的响应。Co胁迫对杨柳幼苗的生长速率、叶绿素含量、可溶性糖含量、光合作用、过氧化物酶活性等生理生化指标均有影响。在2002个差异表达基因（DEGs）中，根特异性差异表达基因1165个，茎和叶特异性差异表达基因837个。DEGs进一步分析表明，在CO胁迫下，在转录组水平上的响应网络中存在多个复杂的级联反应。对氧化胁迫、植物激素信号相关基因和转录因子（TFs）以及过量钴离子解毒反应的详细阐述表明，植物对钴胁迫的防御机制多种多样。我们的研究结果提供了新的和全面的见解植物耐受过量的钴胁迫。
Cobalt (Co) is an essential component of several enzymes and coenzymes in living organisms. Excess Co is highly toxic to plants. The knowledge of molecular response mechanisms to cobalt stress in plants is still limited, especially in woody plants. The responses of weeping willow (Salix babylonica) seedlings to Co stress were studied using morphological and physiochemical measurements and RNA-seq analysis. The physiological and biochemical indexes such as growth rate, the content of chlorophyll and soluble sugar, photosynthesis and peroxidase activity were all changed in willow seedlings under Co stress. The metal ion concentrations in willow including Cu, Zn and Mg were disturbed due to excess Co. Of 2002 differentially expressed genes (DEGs), 1165 were root-specific DEGs and 837 were stem and leaf-specific DEGs. Further analysis of DEGs showed there were multiple complex cascades in the response network at the transcriptome level under Co stress. Detailed elucidation of responses to oxidative stress, phytohormone signaling-related genes and transcription factors (TFs), and detoxification of excess cellular Co ion indicated the various defense mechanisms in plants respond to cobalt stress. Our findings provide new and comprehensive insights into the plant tolerance to excess Co stress.
登录
查看更多内容
影响因子：
5.8
作者：
Tong, Mark Y.;Cassa, Christopher A.;Kohane, Isaac S.
通讯作者：
Kohane, Isaac S.
影响因子：
46.9
作者：
Trapnell C;Williams BA;Pertea G;Mortazavi A;Kwan G;van Baren MJ;Salzberg SL;Wold BJ;Pachter L
通讯作者：
Pachter L
DOI：
10.1016/s0140-6736(80)91745-6
发表时间：
1980-06
期刊：
The Federal Theology of Johannes Cocceius (1603-1669)
影响因子：
--
作者：
George Vogiatzis;Carlos Hernández;R. Cipolla
通讯作者：
George Vogiatzis;Carlos Hernández;R. Cipolla
PlantTFDB 4.0：成为植物转录因子和调控相互作用的中心枢纽。
影响因子：
14.9
作者：
Jin J;Tian F;Yang DC;Meng YQ;Kong L;Luo J;Gao G
通讯作者：
Gao G
影响因子：
5.8
作者：
Simao, Felipe A.;Waterhouse, Robert M.;Zdobnov, Evgeny M.
通讯作者：
Zdobnov, Evgeny M.