Molecular mechanisms of seed dormancy release by gas plasma-activated water technology.
Molecular mechanisms of seed dormancy release by gas plasma-activated water technology.
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DOI：
10.1093/jxb/erac150
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发表时间：
2022-06-24
影响因子：
6.9
通讯作者：
中科院分区：
文献类型：
--
作者：
开发创新农业技术对于全球粮食生产的可持续集约化至关重要。种子休眠是一种适应性特征，它定义了种子能够发芽的环境条件。休眠的释放需要感知和整合多种环境信号，这是一个复杂的过程，可以通过种子处理技术来模仿。在这里，我们揭示了非热（冷）大气等离子体活化水（GPAW）释放拟南芥种子生理休眠的分子机制。 GPAW 通过等离子体产生的活性化学物质（NO3–、H2O2、·NO 和·OH）与针对赤霉素和脱落酸 (ABA) 代谢以及下游细胞壁重塑基因表达的多种信号通路之间的协同相互作用触发休眠释放。 GPAW 对细胞壁的直接化学作用导致胚乳生物力学过早弱化。休眠信号（nlp8、prt6 和dog1）和ABA 代谢（cyp707a2）突变体的萌发反应随GPAW 组成的不同而变化。 GPAW 通过触发多种分子信号传导途径并结合直接化学组织弱化以允许种子萌发，从而消除种子休眠障碍。因此，气体等离子体技术通过模仿宽松的环境来提高种子质量，在该环境中，多个信号的感测和集成导致休眠释放和发芽。气体等离子体活化水农业技术模仿多种环境信号，以增强粮食生产可持续集约化所需的种子发芽和生长。
Developing innovative agri-technologies is essential for the sustainable intensification of global food production. Seed dormancy is an adaptive trait which defines the environmental conditions in which the seed is able to germinate. Dormancy release requires sensing and integration of multiple environmental signals, a complex process which may be mimicked by seed treatment technologies. Here, we reveal molecular mechanisms by which non-thermal (cold) atmospheric gas plasma-activated water (GPAW) releases the physiological seed dormancy of Arabidopsis thaliana. GPAW triggered dormancy release by synergistic interaction between plasma-generated reactive chemical species (NO3–, H2O2, ·NO, and ·OH) and multiple signalling pathways targeting gibberellin and abscisic acid (ABA) metabolism and the expression of downstream cell wall-remodelling genes. Direct chemical action of GPAW on cell walls resulted in premature biomechanical endosperm weakening. The germination responses of dormancy signalling (nlp8, prt6, and dog1) and ABA metabolism (cyp707a2) mutants varied with GPAW composition. GPAW removes seed dormancy blocks by triggering multiple molecular signalling pathways combined with direct chemical tissue weakening to permit seed germination. Gas plasma technologies therefore improve seed quality by mimicking permissive environments in which sensing and integration of multiple signals lead to dormancy release and germination. Gas plasma-activated water agri-technologies mimick the multitude of environmental signals to enhance seed germination and growth required for the sustainable intensification of food production.
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DOI：
10.1073/pnas.1403851111
发表时间：
2014-08-26
影响因子：
11.1
作者：
Graeber, Kai;Linkies, Ada;Leubner-Metzger, Gerhard
通讯作者：
Leubner-Metzger, Gerhard
影响因子：
11.4
作者：
Kushiro, T;Okamoto, M;Nambara, E
通讯作者：
Nambara, E
影响因子：
6.9
作者：
Chen, F;Nonogaki, H;Bradford, KJ
通讯作者：
Bradford, KJ
S-亚硝基化触发 ABI5 降解，促进种子发芽和幼苗生长。
影响因子：
16.6
作者：
Albertos P;Romero-Puertas MC;Tatematsu K;Mateos I;Sánchez-Vicente I;Nambara E;Lorenzo O
通讯作者：
Lorenzo O
影响因子：
3.7
作者：
Bafoil M;Jemmat A;Martinez Y;Merbahi N;Eichwald O;Dunand C;Yousfi M
通讯作者：
Yousfi M