Development of a new pre-vascularized tissue-engineered construct using pre-differentiated rADSCs, arteriovenous vascular bundle and porous nano-hydroxyapatide-polyamide 66 scaffold.
Development of a new pre-vascularized tissue-engineered construct using pre-differentiated rADSCs, arteriovenous vascular bundle and porous nano-hydroxyapatide-polyamide 66 scaffold.
复制标题
使用预分化的 rADSC、动静脉血管束和多孔纳米羟基磷灰石-聚酰胺 66 支架开发新型预血管化组织工程构建体。
DOI：
10.1186/1471-2474-14-318
复制
发表时间：
2013-11-08
影响因子：
2.3
通讯作者：
Wang K
中科院分区：
文献类型：
--
作者：
Yang P;Huang X;Shen J;Wang C;Dang X;Mankin H;Duan Z;Wang K
由于天然骨组织血管网络的复杂性，开发具有用于细胞生长和组织形成的内在血管系统的预血管化组织工程构建体仍然面临许多困难。本研究旨在利用预分化的rADSCs、动静脉血管束和多孔nHA-PA 66支架构建血管化组织工程支架。将rADSC预分化为内皮细胞（rADSCs-Endo），然后在体外掺入nHA-PA 66支架中。随后，按照以下分组进行体内实验：A组（rADSCs-Endo/nHA-PA 66支架带动静脉血管束）、B组（rADSCs-nHA-PA 66支架带动静脉血管束）、C组（nHA-PA 66支架带动静脉血管束）、D组（仅nHA-PA 66支架）。采用组织学和免疫组化方法检测血管密度和血管直径，Western blot检测VEGF-C、FGF-2和BMP-2蛋白表达。体内实验结果显示，A组血管密度和血管直径明显高于其他三组。B组和C组之间，在每个时间点均未观察到统计学差异。结果显示，A组VEGF-C和FGF-2蛋白表达在2周和4周时明显高于B、C和D组。B组和C组之间VEGF-C和FGF-2的表达没有观察到统计学差异。BMP-2在各时间点均不表达。与肌肉包裹法相比，动静脉血管束植入法可促进支架血管化;加入预分化的rADSCs（内皮分化）后，支架血管化速度明显加快。这些阳性结果暗示预分化的rADSCs（内皮分化）与动静脉血管束的结合可以实现生物材料支架的快速血管生成。
Development of a pre-vascularized tissue-engineered construct with intrinsic vascular system for cell growth and tissue formation still faces many difficulties due to the complexity of the vascular network of natural bone tissue. The present study was to design and form a new vascularized tissue-engineered construct using pre-differentiated rADSCs, arteriovenous vascular bundle and porous nHA-PA 66 scaffold. rADSCs were pre-differentiated to endothelial cells (rADSCs-Endo) and then incorporated in nHA-PA 66 scaffolds in vitro. Subsequently, in vivo experiments were carried out according to the following groups: Group A (rADSCs-Endo/nHA-PA 66 scaffold with arteriovenous vascular bundle), Group B (rADSCs/nHA-PA 66 scaffold with arteriovenous vascular bundle); Group C (nHA-PA66 scaffold with arteriovenous vascular bundle), Group D (nHA-PA 66 scaffold only). The vessel density and vessel diameter were measured based on histological and immunohistochemical evaluation, furthermore, the VEGF-C, FGF-2 and BMP-2 protein expressions were also evaluated by western blot analysis. The results of in vivo experiments showed that the vessel density and vessel diameter in group A were significantly higher than the other three groups. Between Group B and C, no statistical difference was observed at each time point. In accordance with the results, there were dramatically higher expressions of VEGF-C and FGF-2 protein in Group A than that of Group B, C and D at 2 or 4 weeks. Statistical differences were not observed in VEGF-C and FGF-2 expression between Group B and C. BMP-2 was not expressed in any group at each time point. Compared with muscular wrapping method, arteriovenous vascular bundle implantation could promote vascularization of the scaffold; and the angiogenesis of the scaffold was significantly accelerated when pre-differentiated rADSCs (endothelial differentiation) were added. These positive results implicate the combination of pre-differentiated rADSCs (endothelial differentiation) and arteriovenous vascular bundle may achieve rapidly angiogenesis of biomaterial scaffold.
登录
查看更多内容
体内可控多孔生物陶瓷材料内部结构与血管化的相关性：定量研究
影响因子：
4.1
作者：
Bai, Feng;Wang, Zhen;Huang, Xin
通讯作者：
Huang, Xin
DOI：
10.1097/01.ta.0000234723.78487.52
发表时间：
2006-11-01
影响因子：
--
作者：
Chen, Alvin Chao-Yu;Chao, En-Kai;Ueng, Steve Wen-Neng
通讯作者：
Ueng, Steve Wen-Neng
影响因子：
--
作者：
Borges, J;Mueller, MC;Stark, GB
通讯作者：
Stark, GB
DOI：
10.1016/j.ijom.2010.01.010
发表时间：
2010-04-01
影响因子：
2.4
作者：
Kokemueller, H.;Spalthoff, S.;Gellrich, N. -C.
通讯作者：
Gellrich, N. -C.
影响因子：
9.7
作者：
Kaushiva, Anchal;Turzhitsky, Vladimir M.;Ameer, Guillermo A.
通讯作者：
Ameer, Guillermo A.