A Multidimensional Mass Spectrometry-Based Workflow for De Novo Structural Elucidation of Oligosaccharides from Polysaccharides.
A Multidimensional Mass Spectrometry-Based Workflow for De Novo Structural Elucidation of Oligosaccharides from Polysaccharides.
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DOI：
10.1021/jasms.1c00133
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发表时间：
2021-08-04
影响因子：
3.2
通讯作者：
Lebrilla CB
中科院分区：
文献类型：
--
作者：
Castillo JJ;Galermo AG;Amicucci MJ;Nandita E;Couture G;Bacalzo N;Chen Y;Lebrilla CB
碳水化合物在各种由其结构决定的生物过程中起着重要作用。然而，表征碳水化合物的结构仍然非常困难，而且通常没有解决。在这项工作中，一种全新的基于质谱的工作流程被开发出来，以分离和结构阐明寡糖，以提供序列、单糖组成和糖苷连接位置。该方法采用基于液相色谱-串联质谱（LC-MS /MS）的三维概念方法：一次高效液相色谱-四极杆飞行时间质谱（HPLC-QTOF MS）分析寡糖测序，两次超高效液相色谱-三重四极杆质谱（UHPLC-QqQ MS）分析分离寡糖以确定其单糖和键的组成。将该方法应用于低聚麦芽糖标准品，验证了该流程的有效性。然后，该方法被用于确定从多糖标准和整个食品中提取的低聚糖的结构。集成的LC-MS工作流程将揭示低聚糖的深入结构。
Carbohydrates play essential roles in a variety of biological processes that are dictated by their structures. However, characterization of carbohydrate structures remains extremely difficult and generally unsolved. In this work, a de novo mass spectrometry-based workflow was developed to isolate and structurally elucidate oligosaccharides to provide sequence, monosaccharide compositions, and glycosidic linkage positions. The approach employs liquid chromatography–tandem mass spectrometry (LC–MS/MS)-based methods in a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis for oligosaccharide sequencing and two ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ MS) analyses on fractionated oligosaccharides to determine their monosaccharides and linkages compositions. The workflow was validated by applying the procedure to maltooligosaccharide standards. The approach was then used to determine the structures of oligosaccharides derived from polysaccharide standards and whole food products. The integrated LC–MS workflow will reveal the in-depth structures of oligosaccharides.
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影响因子：
6.1
作者：
Fureder, Vera;Rodriguez-Colinas, Barbara;Plou, Francisco J.
通讯作者：
Plou, Francisco J.
影响因子：
6.1
作者：
Hogarth, AJCL;Hunter, DE;Wolf, BW
通讯作者：
Wolf, BW
影响因子：
2.3
作者：
Fasciotti, Maira;Sanvido, Gustavo B.;Eberlin, Marcos N.
通讯作者：
Eberlin, Marcos N.
影响因子：
5.6
作者：
Fehlbaum S;Prudence K;Kieboom J;Heerikhuisen M;van den Broek T;Schuren FHJ;Steinert RE;Raederstorff D
通讯作者：
Raederstorff D
影响因子：
5.1
作者：
Etzold, Sabrina;Kober, Olivia I.;Juge, Nathalie
通讯作者：
Juge, Nathalie