Reaction wood - a key cause of variation in cell wall recalcitrance in willow.
Reaction wood - a key cause of variation in cell wall recalcitrance in willow.
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DOI：
10.1186/1754-6834-5-83
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发表时间：
2012-11-22
影响因子：
6.3
通讯作者：
Murphy RJ
中科院分区：
文献类型：
--
作者：
Brereton NJ;Ray MJ;Shield I;Martin P;Karp A;Murphy RJ
在被子植物中，木质纤维素细胞壁生物量对解构的抵抗性差异很大，但这种差异的来源尚不清楚。本文研究了8个短旋矮杨柳（Salix sp.）基因型反应木（RW）响应的变异及其对细胞壁酶促糖化抗性的影响。设计了一项盆栽试验，以测试柳树基因型之间的“RW反应”是否不同，并有助于在田间生长的树木中观察到的细胞壁对酶糖化的抵抗性的差异。通过湿化学测量生物质组成，并与酶糖化的葡萄糖释放量一起测定细胞壁的顽固性。盆栽对照树的葡萄糖释放水平与大田成熟树的葡萄糖释放水平无显著相关。然而，当盆栽树木诱导RW表型时，葡萄糖释放与成熟的田间树木的释放密切相关。实地研究表明，与在更隐蔽的地方生长的相同基因型相比，在高风速（潜在的高RW诱导环境）下生长的基因型的葡萄糖释放量增加了5倍。我们的研究结果为一个新的概念提供了证据，即不同大田生长的柳树基因型（以及潜在的其他被子植物）茎生物量对酶水解的抗性变化。具体来说，基因型差异对RW诱导条件产生反应的能力（“RW反应”）表明，这种RW反应是细胞壁葡聚糖可及性变化的主要决定因素。确定柳树中这种RW响应特性的重要性，可能对柳树（和其他被子植物）生物燃料作物的选择性育种策略有价值，并且随着进一步研究RW变异的性质，可以为改良生物燃料原料的基因改造提供新的目标。
The recalcitrance of lignocellulosic cell wall biomass to deconstruction varies greatly in angiosperms, yet the source of this variation remains unclear. Here, in eight genotypes of short rotation coppice willow (Salix sp.) variability of the reaction wood (RW) response and the impact of this variation on cell wall recalcitrance to enzymatic saccharification was considered. A pot trial was designed to test if the ‘RW response’ varies between willow genotypes and contributes to the differences observed in cell wall recalcitrance to enzymatic saccharification in field-grown trees. Biomass composition was measured via wet chemistry and used with glucose release yields from enzymatic saccharification to determine cell wall recalcitrance. The levels of glucose release found for pot-grown control trees showed no significant correlation with glucose release from mature field-grown trees. However, when a RW phenotype was induced in pot-grown trees, glucose release was strongly correlated with that for mature field-grown trees. Field studies revealed a 5-fold increase in glucose release from a genotype grown at a site exposed to high wind speeds (a potentially high RW inducing environment) when compared with the same genotype grown at a more sheltered site. Our findings provide evidence for a new concept concerning variation in the recalcitrance to enzymatic hydrolysis of the stem biomass of different, field-grown willow genotypes (and potentially other angiosperms). Specifically, that genotypic differences in the ability to produce a response to RW inducing conditions (a ‘RW response’) indicate that this RW response is a primary determinant of the variation observed in cell wall glucan accessibility. The identification of the importance of this RW response trait in willows, is likely to be valuable in selective breeding strategies in willow (and other angiosperm) biofuel crops and, with further work to dissect the nature of RW variation, could provide novel targets for genetic modification for improved biofuel feedstocks.
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影响因子：
6.3
作者：
Brereton NJ;Pitre FE;Ray MJ;Karp A;Murphy RJ
通讯作者：
Murphy RJ
影响因子：
2.4
作者：
Hanley, S. J.;Mallott, M. D.;Karp, A.
通讯作者：
Karp, A.
DOI：
10.1007/s10295-011-0975-y
发表时间：
2011-11-01
影响因子：
3.4
作者：
Munoz, Claudio;Baeza, Jaime;Teixeira Mendonca, Regis
通讯作者：
Teixeira Mendonca, Regis
影响因子：
7.2
作者：
Andersson-Gunnerås, S;Hellgren, JM;Sundberg, B
通讯作者：
Sundberg, B
影响因子：
11.4
作者：
DeMartini, Jaclyn D.;Wyman, Charles E.
通讯作者：
Wyman, Charles E.