Ubiquitin-specific protease 24 negatively regulates abscisic acid signalling in Arabidopsis thaliana.
Ubiquitin-specific protease 24 negatively regulates abscisic acid signalling in Arabidopsis thaliana.
复制标题
泛素特异性蛋白酶 24 负向调节拟南芥中的脱落酸信号传导。
DOI：
10.1111/pce.12628
复制
发表时间：
2016-02
期刊：
影响因子：
--
通讯作者：
Li, Xueyong
中科院分区：
文献类型：
--
作者：
Li, Ming;Yang, Yuhong;Guo, Yan;Li, Xueyong
脱落酸（阿坝）是一种重要的植物激素，参与环境胁迫与植物生长的调控。蛋白质泛素化和去泛素化是分别由E3泛素连接酶和去泛素化酶催化的可逆过程。大量的E3泛素连接酶和经泛素化修饰的转录因子被报道调节阿坝信号转导。然而，到目前为止，还没有发现去泛素化酶在阿坝信号传导中起作用。在这里，我们分离出一个阿坝过度敏感的突变体，ubp 24，其中编码泛素特异性蛋白酶24（UBP 24，At 4g 30890）的基因被破坏的T-DNA插入。ubp 24突变体在萌发后的生长和幼苗生长中对阿坝和盐胁迫都非常敏感。然而，气孔关闭的ubp 24突变体是阿坝的敏感性较低，和ubp 24突变体显示干旱敏感性。UBP 24具有去泛素化酶活性，该活性是UBP 24功能所必需的。此外，UBP 24在体内形成同源二聚体。UBP 24基因位于ABI 2基因的上游，与野生型相比，在阿坝的作用下，UBP 24突变体的蛋白磷酸酶2C的活性降低。这些结果揭示了泛素特异性蛋白酶在植物响应阿坝和盐胁迫中的重要调节作用。
Abscisic acid (ABA) is an important plant hormone integrating environmental stress and plant growth. Protein ubiquitination and deubiquitination are reversible processes catalysed by E3 ubiquitin ligase and deubiquitinating enzyme, respectively. Lots of E3 ubiquitin ligase and transcriptional factors modified by ubiquitination were reported to modulate ABA signalling. However, no deubiquitinating enzyme has been identified that functions in ABA signalling until now. Here, we isolated an ABA overly sensitive mutant, ubp24, in which the gene encoding ubiquitin-specific protease 24 (UBP24, At4g30890) was disrupted by a T-DNA insertion. The ubp24 mutant was hypersensitive to ABA and salt stress in both post-germinative growth and seedling growth. However, stomata closure in the ubp24 mutant was less sensitive to ABA, and the ubp24 mutant showed drought sensitivity. UBP24 possessed deubiquitinating enzyme activity, and the activity was essential for UBP24 function. Additionally, UBP24 formed homodimer in vivo. UBP24 was genetically upstream of ABI2, and the phosphatase activity of protein phosphatase 2C was decreased in the ubp24 mutant compared with the wild type in the presence of ABA. These results uncover an important regulatory role for the ubiquitin-specific protease in response to ABA and salt stress in plant.
登录
查看更多内容
影响因子：
4.4
作者：
Xue, Tongtong;Wang, Dong;Zhang, Shizhong;Ehlting, Juergen;Ni, Fei;Jakab, Stephen;Zheng, Chengchao;Zhong, Yuan
通讯作者：
Zhong, Yuan
DOI：
10.1126/science.1173041
发表时间：
2009-05-22
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Park SY;Fung P;Nishimura N;Jensen DR;Fujii H;Zhao Y;Lumba S;Santiago J;Rodrigues A;Chow TF;Alfred SE;Bonetta D;Finkelstein R;Provart NJ;Desveaux D;Rodriguez PL;McCourt P;Zhu JK;Schroeder JI;Volkman BF;Cutler SR
通讯作者：
Cutler SR
DOI：
10.1002/j.1460-2075.1987.tb02730.x
发表时间：
1987-12
期刊：
The EMBO Journal
影响因子：
--
作者：
R. Jefferson;T. Kavanagh;M. Bevan
通讯作者：
R. Jefferson;T. Kavanagh;M. Bevan
影响因子：
11.6
作者：
Liu, Hongxia;Stone, Sophia L.
通讯作者：
Stone, Sophia L.
ABO3 是一种 WRKY 转录因子，介导拟南芥植物对脱落酸的反应和耐旱性。
DOI：
10.1111/j.1365-313x.2010.04248.x
发表时间：
2010-08
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Ren X;Chen Z;Liu Y;Zhang H;Zhang M;Liu Q;Hong X;Zhu JK;Gong Z
通讯作者：
Gong Z