Casein Hydrolysate Alleviates Adipose Chronic Inflammation in High Fat-Diet Induced Obese C57BL/6J Mice through MAPK Pathway.
Casein Hydrolysate Alleviates Adipose Chronic Inflammation in High Fat-Diet Induced Obese C57BL/6J Mice through MAPK Pathway.
复制标题
酪蛋白水解物通过MAPK途径减轻高脂饮食诱导的肥胖C57 BL/6 J小鼠脂肪慢性炎症
作者：
Liu L;Yu S;Bu T;He G;Li S;Wu J
肥胖引起的脂肪慢性炎症与胰岛素抵抗和2型糖尿病的发生密切相关。牛酪蛋白衍生的三肽L-缬氨酰-L-脯氨酰-L-脯氨酸（VPP）和L-异亮氨酰-L-脯氨酰-L-脯氨酸（IPP）已被报道可预防脂肪细胞的炎症变化并减轻胰岛素抵抗。本研究旨在探讨酪蛋白水解物（CH）对高脂饮食（HFD）诱导的肥胖小鼠和细胞因子TNF-α诱导的脂肪细胞的影响。我们的数据表明，CH减轻慢性炎症在体内和体外。4% CH抑制HFD诱导的全身炎症因子、肥大白色脂肪细胞和巨噬细胞浸润。更重要的是，CH能够通过增加CCAAT/增强子结合蛋白α（C/EBP-α）而不是过氧化物酶体增殖物激活受体γ（PPAR-γ）的表达来改善TNF-α诱导的脂肪细胞功能障碍。此外，CH还剂量依赖性地抑制TNF-α诱导的3 T3-L1细胞中丝裂原活化蛋白激酶（MAPK）-c-Jun N-末端激酶（JNK）磷酸化，并增强Erk 1/2的磷酸化，但不增强核因子-κ B（NF-κB）p65的磷酸化。以上结果表明，CH可通过MAPK途径减轻脂肪慢性炎症。总之，我们的研究结果表明，补充4% CH 6周对预防肥胖相关炎症和脂肪功能障碍具有保护作用。
Obesity-induced adipose chronic inflammation is closely related to the development of insulin resistance and T2DM. Tripeptides l-valyl-l-prolyl-l-proline (VPP) and l-isoleucyl-l-prolyl-L-proline (IPP) derived from bovine casein have been reported to prevent inflammatory changes and mitigate insulin resistance in adipocytes. In this study, we aimed to investigate the influence of casein hydrolysates (CH) containing VPP and IPP on a high fat diet (HFD)-induced obese mice and cytokine TNF-α-induced adipocytes. Our data showed that CH alleviated chronic inflammation both in vivo and in vitro. 4% CH suppressed HFD-induced systemic inflammatory factors, hypertrophic white adipocytes, and macrophage infiltration. More importantly, CH was able to improve adipocyte dysfunction induced by TNF-α by increasing the expression of CCAAT/enhancer binding protein α (C/EBP-α) rather than peroxisome proliferator-activated receptor γ (PPAR-γ). Furthermore, CH also dose-dependently suppressed mitogen-activated protein kinase (MAPK)-c-Jun N-terminal kinase (JNK) phosphorylation and enhanced the phosphorylation of Erk 1/2, but not nuclear factor-kappa B (NF-κB) p65 phosphorylation, in TNF-α-induced 3T3-L1 cells. These results indicated that CH could ameliorate adipose chronic inflammation through the MAPK pathway. Altogether, our findings suggested that 4% CH supplementation for 6 weeks exerted a protective role in preventing obesity-related inflammation and adipose dysfunction.
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影响因子：
3.6
作者：
Aihara, Kotaro;Osaka, Mizuko;Yoshida, Masayuki
通讯作者：
Yoshida, Masayuki
影响因子：
7
作者：
Coppack, SW
通讯作者：
Coppack, SW
影响因子：
6.1
作者：
Chakrabarti, Subhadeep;Jahandideh, Forough;Wu, Jianping
通讯作者：
Wu, Jianping
影响因子：
3.7
作者：
Hagita S;Osaka M;Shimokado K;Yoshida M
通讯作者：
Yoshida M
DOI：
10.1016/s0140-6736(16)30054-x
发表时间：
2016-04-02
期刊：
Lancet (London, England)
影响因子：
--
作者：
NCD Risk Factor Collaboration (NCD-RisC)
通讯作者：
NCD Risk Factor Collaboration (NCD-RisC)