Virological and serological findings in dogs with naturally occurring distemper.

Virological and serological findings in dogs with naturally occurring distemper.
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DOI:
10.1016/j.jviromet.2014.12.004
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发表时间:
2015-03
影响因子:
3.1
通讯作者:
Buonavoglia C
Buonavoglia C
中科院分区:
医学4区
文献类型:
--
作者:
Elia G;Camero M;Losurdo M;Lucente MS;Larocca V;Martella V;Decaro N;Buonavoglia C

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采集合适的样本对犬瘟热(CD)的诊断至关重要。尿液是诊断CD最合适的标本。CD的全球流行病学加强了准确诊断的重要性。犬瘟热病毒(CDV)是一种严重且高度传染性的犬类疾病的病原体。CDV相关疾病的不可预测和多变的病程可能会阻碍对感染的正确诊断,并使收集适合实验室确认的样本变得至关重要。在目前的研究中,我们能够跟踪两只自然感染的狗的疾病,在整个感染期间收集不同的生物基质。通过实时定量RT-PCR对病毒RNA进行检测和定量,表明无论临床分期和疾病形式如何,尿液和直肠拭子都可以用于犬瘟热的死前诊断。
The collection of suitable samples are crucial for Canine Distemper (CD) diagnosis. Urine proved to be the most suitable sample for CD diagnosis. The global epidemiology of CD reinforces the importance of an accurate diagnostic. Canine distemper virus (CDV) is the cause of a severe and highly contagious disease in dogs. The unpredictable and variable course of CDV-related disease may hamper correct diagnosis of infection and makes it crucial the collection of samples suitable for laboratory confirmation. In the present study we were able to follow the disease in two dogs infected naturally, collecting different biological matrices during the entire period of infection. By real time RT-PCR, viral RNA was detected and quantified, suggesting that urine and rectal swabs would be useful for ante-mortem diagnosis of distemper in dogs, regardless of the clinical stage and form of the illness.
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