The role of the mucin-glycan foraging Ruminococcus gnavus in the communication between the gut and the brain.
The role of the mucin-glycan foraging Ruminococcus gnavus in the communication between the gut and the brain.
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DOI：
10.1080/19490976.2022.2073784
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发表时间：
2022-01
期刊：
影响因子：
12.2
通讯作者：
Juge, Nathalie
中科院分区：
文献类型：
--
作者：
Coletto, Erika;Latousakis, Dimitrios;Pontifex, Matthew G.;Crost, Emmanuelle H.;Vaux, Laura;Santamarina, Estella Perez;Goldson, Andrew;Brion, Arlaine;Hajihosseini, Mohammad K.;Vauzour, David;Savva, George M.;Juge, Nathalie
关键词：
活泼瘤胃球菌（Ruminococcus gavus）是人类肠道微生物群的普遍成员，其在炎症性肠病和神经系统疾病中过度存在。我们以前证明了R. gavus觅食粘蛋白是应变依赖性的，并与唾液酸代谢。在这里，我们发现，小鼠单殖民与R。gavus ATCC 29149（Rg-小鼠）在其盲肠内容物、血液和脑中显示主要唾液酸衍生物的变化，这伴随着相对于无菌（GF）小鼠肠粘蛋白中唾液酸化残基百分比的显著降低。与GF小鼠相比，在Rg小鼠的盲肠内容物中也检测到与脑功能相关的代谢物如色胺、吲哚乙酸盐和三甲胺N-氧化物的变化。接下来，我们研究了R. gavus单克隆对海马细胞增殖和行为的影响。我们观察到一个显着的减少PSA-NCAM免疫反应颗粒细胞在齿状回（DG）的RG-小鼠相比，GF小鼠和附近的吞噬小胶质细胞的招聘。行为评估表明Rg小鼠的空间工作记忆得到改善，但其他认知功能没有变化。参与增殖和神经可塑性的基因的显著上调也支持这些结果。总的来说，这些数据提供了关于R。gnavus代谢物可能通过调节成年海马中的颗粒细胞发育和突触可塑性来影响脑调节和功能。这项工作对进一步理解R的作用机制具有重要意义。神经系统疾病中的痣。
Ruminococcus gnavus is a prevalent member of the human gut microbiota, which is over-represented in inflammatory bowel disease and neurological disorders. We previously showed that the ability of R. gnavus to forage on mucins is strain-dependent and associated with sialic acid metabolism. Here, we showed that mice monocolonized with R. gnavus ATCC 29149 (Rg-mice) display changes in major sialic acid derivatives in their cecum content, blood, and brain, which is accompanied by a significant decrease in the percentage of sialylated residues in intestinal mucins relative to germ-free (GF) mice. Changes in metabolites associated with brain function such as tryptamine, indolacetate, and trimethylamine N-oxide were also detected in the cecal content of Rg-mice when compared to GF mice. Next, we investigated the effect of R. gnavus monocolonization on hippocampus cell proliferation and behavior. We observed a significant decrease of PSA-NCAM immunoreactive granule cells in the dentate gyrus (DG) of Rg-mice as compared to GF mice and recruitment of phagocytic microglia in the vicinity. Behavioral assessments suggested an improvement of the spatial working memory in Rg-mice but no change in other cognitive functions. These results were also supported by a significant upregulation of genes involved in proliferation and neuroplasticity. Collectively, these data provide first insights into how R. gnavus metabolites may influence brain regulation and function through modulation of granule cell development and synaptic plasticity in the adult hippocampus. This work has implications for further understanding the mechanisms underpinning the role of R. gnavus in neurological disorders.
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影响因子：
3.3
作者：
Davari, S.;Talaei, S. A.;Salami, M.
通讯作者：
Salami, M.
影响因子：
15.5
作者：
D'Amato A;Di Cesare Mannelli L;Lucarini E;Man AL;Le Gall G;Branca JJV;Ghelardini C;Amedei A;Bertelli E;Regoli M;Pacini A;Luciani G;Gallina P;Altera A;Narbad A;Gulisano M;Hoyles L;Vauzour D;Nicoletti C
通讯作者：
Nicoletti C
DOI：
10.1074/jbc.ra120.014454
发表时间：
2020-10-02
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Bell A;Severi E;Lee M;Monaco S;Latousakis D;Angulo J;Thomas GH;Naismith JH;Juge N
通讯作者：
Juge N
影响因子：
3.7
作者：
Crost EH;Tailford LE;Le Gall G;Fons M;Henrissat B;Juge N
通讯作者：
Juge N
影响因子：
3.7
作者：
Attar A;Liu T;Chan WT;Hayes J;Nejad M;Lei K;Bitan G
通讯作者：
Bitan G