Utilisation of mucin glycans by the human gut symbiont Ruminococcus gnavus is strain-dependent.
Utilisation of mucin glycans by the human gut symbiont Ruminococcus gnavus is strain-dependent.
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DOI：
10.1371/journal.pone.0076341
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Juge N
中科院分区：
文献类型：
--
作者：
Crost EH;Tailford LE;Le Gall G;Fons M;Henrissat B;Juge N
共生细菌通常具有特别丰富的聚糖降解酶来源，这使得它们能够利用食物或宿主中未消化的碳水化合物。活泼瘤胃球菌（Ruminococcus gavus）存在于≥90%的人类消化道中，并与肠道相关疾病如炎症性肠病（IBD）有关。本文分析了两个R. gnavus人菌株E1和ATCC 29149，以利用宿主聚糖。结果表明，虽然两株菌都能同化粘蛋白单糖，但只有R。gnavus ATCC 29149能够在作为唯一碳源的粘蛋白上生长。对这两个R. gnavus菌株突出了潜在的簇和糖苷水解酶（GH），其负责粘蛋白衍生的聚糖的分解和利用。转录组学和功能活性测定证实了特异性GH33唾液酸酶以及GH29和GH95岩藻糖苷酶在粘蛋白利用途径中的重要性。值得注意的是，我们发现了一个新的途径，通过该途径，R。gavus ATCC 29149利用来自唾液酸化底物的唾液酸。我们的结果也证明了R. gavus ATCC 29149中的菌株，当在粘蛋白和岩藻糖基化聚糖上生长时，产生丙醇和丙酸盐作为代谢的终产物。这些新的发现为R. gnavus适应肠道环境推进我们的理解肠道微生物在健康和疾病中的作用。
Commensal bacteria often have an especially rich source of glycan-degrading enzymes which allow them to utilize undigested carbohydrates from the food or the host. The species Ruminococcus gnavus is present in the digestive tract of ≥90% of humans and has been implicated in gut-related diseases such as inflammatory bowel diseases (IBD). Here we analysed the ability of two R. gnavus human strains, E1 and ATCC 29149, to utilize host glycans. We showed that although both strains could assimilate mucin monosaccharides, only R. gnavus ATCC 29149 was able to grow on mucin as a sole carbon source. Comparative genomic analysis of the two R. gnavus strains highlighted potential clusters and glycoside hydrolases (GHs) responsible for the breakdown and utilization of mucin-derived glycans. Transcriptomic and functional activity assays confirmed the importance of specific GH33 sialidase, and GH29 and GH95 fucosidases in the mucin utilisation pathway. Notably, we uncovered a novel pathway by which R. gnavus ATCC 29149 utilises sialic acid from sialylated substrates. Our results also demonstrated the ability of R. gnavus ATCC 29149 to produce propanol and propionate as the end products of metabolism when grown on mucin and fucosylated glycans. These new findings provide molecular insights into the strain-specificity of R. gnavus adaptation to the gut environment advancing our understanding of the role of gut commensals in health and disease.
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DOI：
10.1096/fj.12-221416
发表时间：
2013-06
期刊：
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
影响因子：
--
作者：
Gunning AP;Kirby AR;Fuell C;Pin C;Tailford LE;Juge N
通讯作者：
Juge N
影响因子：
64.8
作者：
Arumugam, Manimozhiyan;Raes, Jeroen;Pelletier, Eric;Le Paslier, Denis;Yamada, Takuji;Mende, Daniel R.;Fernandes, Gabriel R.;Tap, Julien;Bruls, Thomas;Batto, Jean-Michel;Bertalan, Marcelo;Borruel, Natalia;Casellas, Francesc;Fernandez, Leyden;Gautier, Laurent;Hansen, Torben;Hattori, Masahira;Hayashi, Tetsuya;Kleerebezem, Michiel;Kurokawa, Ken;Leclerc, Marion;Levenez, Florence;Manichanh, Chaysavanh;Nielsen, H. Bjorn;Nielsen, Trine;Pons, Nicolas;Poulain, Julie;Qin, Junjie;Sicheritz-Ponten, Thomas;Tims, Sebastian;Torrents, David;Ugarte, Edgardo;Zoetendal, Erwin G.;Wang, Jun;Guarner, Francisco;Pedersen, Oluf;de Vos, Willem M.;Brunak, Soren;Dore, Joel;Weissenbach, Jean;Ehrlich, S. Dusko;Bork, Peer
通讯作者：
Bork, Peer
影响因子：
24.5
作者：
Joossens, Marie;Huys, Geert;Vermeire, Severine
通讯作者：
Vermeire, Severine
影响因子：
6.8
作者：
Henrissat, B;Davies, G
通讯作者：
Davies, G
影响因子：
3.4
作者：
Almagro-Moreno S;Boyd EF
通讯作者：
Boyd EF