Recovery of Amiloride-Sensitive Neural Coding during Regeneration of the Gustatory Nerve: Behavioral–Neural Correlation of Salt Taste Discrimination
Recovery of Amiloride-Sensitive Neural Coding during Regeneration of the Gustatory Nerve: Behavioral–Neural Correlation of Salt Taste Discrimination
复制标题
味觉神经再生过程中阿米洛利敏感神经编码的恢复：盐味辨别的行为神经相关性
DOI：
10.1523/jneurosci.23-10-04362.2003
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Y. Ninomiya
中科院分区：
文献类型：
--
作者：
K. Yasumatsu;H. Katsukawa;K. Sasamoto;Y. Ninomiya
支配舌前部的鼓索（CT）神经包含两种类型的NaCl响应纤维：一种是N型，接收来自受体细胞的输入，其NaCl响应被阿米洛利强烈抑制，而另一种是E型，接收来自对阿米洛利不敏感或不敏感的细胞的输入。为了研究这种差异反应神经系统的形成，我们压碎了小鼠CT神经，并检查了随后的恢复NaCl反应和再生神经的阿米洛利敏感性以及NaCl和KCl之间的行为辨别。在神经挤压后2周，未观察到神经对化学刺激的显著反应。在3周时，对盐的反应逐渐重现。在此期间，几乎所有的单纤维响应NaCl阿米洛利不敏感（E型）。4周时，部分单纤维表现出阿米洛利敏感性（N型），并再次出现对NaCl和KCl的行为辨别。≥5周后，N型纤维的数量已达到对照水平，并与E型纤维的数量大致相等。在恢复的过程中，N型和E型纤维的阿米洛利的敏感性，KCl/NaCl的响应比，和他们的浓度响应关系NaCl的基础上，明确区分。这些结果表明，两个盐响应系统的独立改造后，神经夹伤。这种选择性突触重建可能是味神经损伤后再再生时，对NaCl和KCl的行为辨别能力得以恢复的原因。这也可以解释在健康动物的感受器细胞的连续周转过程中，味觉质量的稳定感觉编码。
The chorda tympani (CT) nerve innervating the anterior tongue contains two types of NaCl-responsive fibers: one, the N-type, receives input from receptor cells, the NaCl responses of which are strongly inhibited by amiloride, whereas the other, the E-type, receives input from cells poorly sensitive or insensitive to amiloride. To investigate the formation of this differentially responsive neural system, we crushed the mouse CT nerve and examined the subsequent recovery of NaCl responses and amiloride sensitivity of the regenerated nerve and behavioral discrimination between NaCl and KCl. At 2 weeks after the nerve crush, no significant response of the nerve to chemical stimuli was observed. At 3 weeks, responses to salts gradually reappeared. In this period, almost all single fibers responding to NaCl were insensitive to amiloride (E-type). At 4 weeks, some of the single fibers showed amiloride sensitivity (N-type) and behavioral discrimination between NaCl and KCl reappeared. After ≥5 weeks, the number of N-type fibers had reached the control level and became approximately equal to that of E-type fibers. During the course of recovery, N-type and E-type fibers were clearly distinguishable on the basis of their amiloride sensitivities, their KCl/NaCl response ratios, and their concentration–response relationships to NaCl. These results suggest that two salt-responsive systems are independently reformed after the nerve crush. The selective synapse reformation may account for recovery of behavioral discrimination between NaCl and KCl after taste nerve crush and regeneration. It may also explain stable sensory coding for taste quality during the continuous turnover of receptor cells in the healthy animal.
影响因子：
56.9
作者：
HECK, GL;MIERSON, S;DESIMONE, JA
通讯作者：
DESIMONE, JA
盐辨别能力与大鼠鼓索神经切断后再生味蕾的数量有关。
DOI：
10.1152/ajpregu.1995.269.1.r141
发表时间：
1995
期刊：
The American journal of physiology.
影响因子：
--
作者：
StJohn,SJ;Markison,S;Spector,AC
通讯作者：
Spector,AC