Trauma-induced alterations in cognition and Arc expression are reduced by previous exposure to 56Fe irradiation.
Trauma-induced alterations in cognition and Arc expression are reduced by previous exposure to 56Fe irradiation.
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DOI：
10.1002/hipo.20920
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发表时间：
2012-03
期刊：
影响因子：
3.5
通讯作者：
Fike, John R.
中科院分区：
文献类型：
--
作者：
Rosi, Susanna;Belarbi, Karim;Ferguson, Ryan A.;Fishman, Kelly;Obenaus, Andre;Raber, Jacob;Fike, John R.
暴露于电离辐射可能直接影响脑功能，但也可能改变组织对二次损伤（如创伤、中风或退行性疾病）的敏感性。为了确定低剂量的颗粒辐射是否使脑对随后的损伤敏感，将C56 BL 6小鼠暴露于用0.5戈伊的56 Fe离子进行的仅脑辐射。两个月后，使用可控皮质撞击系统诱导单侧创伤性脑损伤。创伤后三周，动物接受多次BrdU注射，30天后在Morris水迷宫中测试认知性能。在训练期间，所有动物都能够定位可见和隐藏的平台;然而，当在探索试验中评估空间记忆保持时，观察到治疗效果（无平台）。虽然假手术和辐射的动物表现出空间记忆保留，但单独接受创伤的小鼠没有。当创伤之前的辐射，在水迷宫中的性能是没有不同的假治疗的动物，这表明低剂量辐射的背景下，随后的创伤性损伤的保护作用。海马神经发生的措施表明，复合损伤并没有引起任何变化更大的创伤或辐射后看到的。创伤后，在两个半球中表达行为诱导的立即早期基因Arc的神经元的百分比显著降低，而没有相关的神经元损失。联合损伤后，相对于假手术治疗的小鼠没有差异。我们的研究结果表明，与单独创伤相比，联合损伤导致我们的终点改变减少。虽然潜在的机制尚不清楚，但这些结果类似于预处理、适应性或诱导样保护性反应，其中亚致死或潜在伤害性刺激（即辐照）诱导对随后的和潜在更具破坏性的损伤（创伤）的耐受。
Exposure to ionizing irradiation may affect brain functions directly, but may also change tissue sensitivity to a secondary insult such as trauma, stroke or degenerative disease. To determine if a low dose of particulate irradiation sensitizes the brain to a subsequent injury, C56BL6 mice were exposed to brain only irradiation with 0.5 Gy of 56Fe ions. Two months later, unilateral traumatic brain injury was induced using a controlled cortical impact system. Three weeks after trauma animals received multiple BrdU injections and 30 days later were tested for cognitive performance in the Morris water maze. All animals where able to locate the visible and hidden platform during training; however, treatment effects were seen when spatial memory retention was assessed in the probe trial (no platform). While sham and irradiated animals showed spatial memory retention, mice that received trauma alone did not. When trauma was preceded by irradiation, performance in the water maze was not different from sham-treated animals, suggesting that low dose irradiation had a protective effect in the context of a subsequent traumatic injury. Measures of hippocampal neurogenesis showed that combined injury did not induce any changes greater that those seen after trauma or radiation alone. After trauma there was a significant decrease in the percentage of neurons expressing the behaviorally-induced immediate early gene Arc in both hemispheres, without associated neuronal loss. After combined injury there were no differences relative to sham-treated mice. Our results suggest that combined injury resulted in decreased alterations of our endpoints compared to trauma alone. While the underlying mechanisms are not yet known, these results resemble a preconditioning, adaptive, or inducible-like protective response, where a sublethal or potentially injurious stimulus (i.e. irradiation) induces tolerance to a subsequent and potentially more damaging insult (trauma).
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作者：
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