Thermal difference spectra: a specific signature for nucleic acid structures.
Thermal difference spectra: a specific signature for nucleic acid structures.
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DOI：
10.1093/nar/gni134
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发表时间：
2005-09-12
影响因子：
14.9
通讯作者：
Chaires JB
中科院分区：
文献类型：
--
作者：
Mergny JL;Li J;Lacroix L;Amrane S;Chaires JB
我们表明，核酸结构可以方便和廉价的特点是他们的紫外热差光谱。通过简单地记录核酸在高于和低于其解链温度（Tm）的温度下未折叠和折叠状态的紫外吸收光谱，获得核酸的热差光谱（TDS）。这两个光谱之间的差异是TDS。TDS具有特定的形状，对于每种类型的核酸结构都是独特的，这一结论是基于对来自200个不同序列的>900个光谱的比较。TDS的形状反映了每种类型的核酸结构中独特发生的碱基堆积相互作用的微妙之处。TDS提供了一种简单、廉价和快速的方法来获得对核酸结构的结构洞察，其适用于从短寡聚体到多核苷酸的DNA和RNA。TDS补充圆二色性作为一种工具的核酸在溶液中的结构表征。
We show that nucleic acid structures may be conveniently and inexpensively characterized by their UV thermal difference spectra. A thermal difference spectrum (TDS) is obtained for a nucleic acid by simply recording the ultraviolet absorbance spectra of the unfolded and folded states at temperatures above and below its melting temperature (Tm). The difference between these two spectra is the TDS. The TDS has a specific shape that is unique for each type of nucleic acid structure, a conclusion that is based on a comparison of >900 spectra from 200 different sequences. The shape of the TDS reflects the subtleties of base stacking interactions that occur uniquely within each type of nucleic acid structure. TDS provides a simple, inexpensive and rapid method to obtain structural insight into nucleic acid structures, which is applicable to both DNA and RNA from short oligomers to polynucleotides. TDS complements circular dichroism as a tool for the structural characterization of nucleic acids in solution.
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