More Than One-to-Four via 2R: Evidence of an Independent Amphioxus Expansion and Two-Gene Ancestral Vertebrate State for MyoD-Related Myogenic Regulatory Factors (MRFs).
More Than One-to-Four via 2R: Evidence of an Independent Amphioxus Expansion and Two-Gene Ancestral Vertebrate State for MyoD-Related Myogenic Regulatory Factors (MRFs).
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DOI：
10.1093/molbev/msaa147
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发表时间：
2020-10-01
影响因子：
10.7
通讯作者：
Ferrier DEK
中科院分区：
文献类型：
--
作者：
Aase-Remedios ME;Coll-Lladó C;Ferrier DEK
从无脊椎动物到脊椎动物的进化过渡涉及广泛的基因复制，但要准确理解这种复制是如何促成这种过渡的，需要对基因和基因家族的具体情况有更详细的了解。肌源性分化（MyoD）长期以来被认为是一个主要的发育控制基因，也是驱动两侧肌生成的bHLH转录因子（肌源性调节因子[MRF]）MyoD家族的成员。在这个基因家族内的系统发育重建是复杂的基因重复和损失在几个谱系的多个实例。在脊椎动物起源的两轮全基因组复制（2 R WGD）之后，MRF的祖先功能被认为已经在子代基因之间分配，因此MyoD和Myf 5在肌生成决定中早期起作用，而Myog和Myf 6在分化成肌细胞中表达较晚。比较脊索动物的MRFs，我们发现一个独立的扩展MRFs在无脊椎动物脊索动物文昌鱼，与证据的一个平行的例子，相对于脊椎动物的亚功能化。脊索动物MRF基因座之间的保守同线性支持2 R WGD事件作为塑造脊椎动物MRF进化的主要力量。我们还解决了脊椎动物MRF的补充和组织，在这个过程中发现了一种新的脊椎动物MRF基因，这使我们能够推断出脊椎动物中对应于早期和晚期作用型MRF的祖先两个基因状态。这就需要对以前关于脊椎动物MRF简单的1 - 4起源的结论进行修订。
The evolutionary transition from invertebrates to vertebrates involved extensive gene duplication, but understanding precisely how such duplications contributed to this transition requires more detailed knowledge of specific cases of genes and gene families. Myogenic differentiation (MyoD) has long been recognized as a master developmental control gene and member of the MyoD family of bHLH transcription factors (myogenic regulatory factors [MRFs]) that drive myogenesis across the bilaterians. Phylogenetic reconstructions within this gene family are complicated by multiple instances of gene duplication and loss in several lineages. Following two rounds of whole-genome duplication (2R WGD) at the origin of the vertebrates, the ancestral function of MRFs is thought to have become partitioned among the daughter genes, so that MyoD and Myf5 act early in myogenic determination, whereas Myog and Myf6 are expressed later, in differentiating myoblasts. Comparing chordate MRFs, we find an independent expansion of MRFs in the invertebrate chordate amphioxus, with evidence for a parallel instance of subfunctionalization relative to that of vertebrates. Conserved synteny between chordate MRF loci supports the 2R WGD events as a major force in shaping the evolution of vertebrate MRFs. We also resolve vertebrate MRF complements and organization, finding a new type of vertebrate MRF gene in the process, which allowed us to infer an ancestral two-gene state in the vertebrates corresponding to the early- and late-acting types of MRFs. This necessitates a revision of previous conclusions about the simple one-to-four origin of vertebrate MRFs.
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影响因子：
14.9
作者：
Cameron RA;Samanta M;Yuan A;He D;Davidson E
通讯作者：
Davidson E
DOI：
10.1073/pnas.1610605114
发表时间：
2017-06-06
影响因子：
11.1
作者：
Buckingham, Margaret
通讯作者：
Buckingham, Margaret
DOI：
10.1073/pnas.1014235108
发表时间：
2011-05-31
影响因子：
11.1
作者：
Bertrand, Stephanie;Camasses, Alain;Escriva, Hector
通讯作者：
Escriva, Hector
影响因子：
10.5
作者：
Carvajal, Jaime J.;Keith, Annette;Rigby, Peter W. J.
通讯作者：
Rigby, Peter W. J.
影响因子：
12.3
作者：
Dalgleish, Raymond;Flicek, Paul;Maglott, Donna R.
通讯作者：
Maglott, Donna R.