Mucin Transiently Sustains Coronavirus Infectivity through Heterogenous Changes in Phase Morphology of Evaporating Aerosol.
Mucin Transiently Sustains Coronavirus Infectivity through Heterogenous Changes in Phase Morphology of Evaporating Aerosol.
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DOI：
10.3390/v14091856
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发表时间：
2022-08-24
期刊：
影响因子：
--
通讯作者：
Reid JP
中科院分区：
文献类型：
--
作者：
Alexander RW;Tian J;Haddrell AE;Oswin HP;Neal E;Hardy DA;Otero-Fernandez M;Mann JFS;Cogan TA;Finn A;Davidson AD;Hill DJ;Reid JP
呼吸道病原体可通过雾化呼气分泌物以飞沫或微粒的形式传播。了解控制这些病原体在空气传播时如何存活的基本气溶胶参数，对于了解和制定限制其传播的方法至关重要。病原体活性测量使用受控电动悬浮和将生物气溶胶提取到底物(CREBS)，与比较动力学电动平衡(CKEDB)测量相结合，可以直接比较病毒活性和气溶胶的蒸发动力学，时间分辨率为秒。在这里，我们报告了小鼠肝炎病毒(MHV)在空气中的存活情况，并确定了与我们之前观察到的严重急性呼吸综合征冠状病毒2(SARS-CoV-2)类似的气溶胶阶段的传染性丧失。通过将临床相关浓度的粘蛋白添加到生物气雾剂中，在相对湿度为40%时，病毒感染性的丧失有一过性的缓解。粘蛋白浓度的增加促进了气溶胶蒸发过程中的不均匀相变，其特征是在宿主液滴中形成包裹体。这项研究证明了粘液在气溶胶阶段的作用及其对短期空气传播病毒稳定性的影响。
Respiratory pathogens can be spread though the transmission of aerosolised expiratory secretions in the form of droplets or particulates. Understanding the fundamental aerosol parameters that govern how such pathogens survive whilst airborne is essential to understanding and developing methods of restricting their dissemination. Pathogen viability measurements made using Controlled Electrodynamic Levitation and Extraction of Bioaerosol onto Substrate (CELEBS) in tandem with a comparative kinetics electrodynamic balance (CKEDB) measurements allow for a direct comparison between viral viability and evaporation kinetics of the aerosol with a time resolution of seconds. Here, we report the airborne survival of mouse hepatitis virus (MHV) and determine a comparable loss of infectivity in the aerosol phase to our previous observations of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Through the addition of clinically relevant concentrations of mucin to the bioaerosol, there is a transient mitigation of the loss of viral infectivity at 40% RH. Increased concentrations of mucin promoted heterogenous phase change during aerosol evaporation, characterised as the formation of inclusions within the host droplet. This research demonstrates the role of mucus in the aerosol phase and its influence on short-term airborne viral stability.
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DOI：
10.1073/pnas.2109750119
发表时间：
2022-01-25
影响因子：
11.1
作者：
Huynh E;Olinger A;Woolley D;Kohli RK;Choczynski JM;Davies JF;Lin K;Marr LC;Davis RD
通讯作者：
Davis RD
DOI：
10.1056/nejmra0910061
发表时间：
2010-12-02
期刊：
The New England journal of medicine
影响因子：
--
作者：
Fahy JV;Dickey BF
通讯作者：
Dickey BF
影响因子：
--
作者：
Leibowitz, Julian;Kaufman, Gili;Liu, Pinghua
通讯作者：
Liu, Pinghua
影响因子：
3
作者：
CHAUNCEY, HH;LEVINE, DM;KULCZYEKI, LL
通讯作者：
KULCZYEKI, LL
DOI：
10.1056/nejmoa1701632
发表时间：
2017-09-07
期刊：
The New England journal of medicine
影响因子：
--
作者：
Kesimer M;Ford AA;Ceppe A;Radicioni G;Cao R;Davis CW;Doerschuk CM;Alexis NE;Anderson WH;Henderson AG;Barr RG;Bleecker ER;Christenson SA;Cooper CB;Han MK;Hansel NN;Hastie AT;Hoffman EA;Kanner RE;Martinez F;Paine R 3rd;Woodruff PG;O'Neal WK;Boucher RC
通讯作者：
Boucher RC