Determination of Hepatic Iron Deposition in Drug-Induced Liver Fibrosis in Rats by Confocal Micro-XRF Spectrometry.
Determination of Hepatic Iron Deposition in Drug-Induced Liver Fibrosis in Rats by Confocal Micro-XRF Spectrometry.
复制标题
共焦微型 XRF 光谱法测定大鼠药物性肝纤维化中的肝铁沉积
DOI：
10.1021/acsomega.1c06476
复制
发表时间：
2022-02-01
期刊：
影响因子：
4.1
通讯作者：
Li F
中科院分区：
文献类型：
--
作者：
Xu Q;Xia W;Zhou L;Zou Z;Li Q;Deng L;Wu S;Wang T;Cui J;Liu Z;Sun T;Ye J;Li F
肝纤维化是慢性肝病发展为肝硬化的中间过程和必然阶段。降低肝纤维化程度对治疗慢性肝病、预防肝硬化和肝癌具有极其重要的作用。肝纤维化的形成在一定程度上受到铁沉积的影响，过量的铁沉积进一步诱发肝硬化和肝癌。采用微束共聚焦X射线荧光（μ-XRF）技术，观察硫代乙酰胺（TAA）诱导大鼠肝纤维化过程中不同时间点铁沉积的强度和生物分布。据我们所知，这是第一项使用共焦μ-XRF分析肝纤维化中肝脏铁沉积的研究。结果表明，大鼠肝脏中含有铁、钾、锌等微量元素。连续注射TAA溶液导致肝脏铁沉积随着时间的推移而增加。注射骨髓间充质干细胞后，肝组织铁沉积的强度也显著降低。结果表明，共焦μ-XRF可作为一种非破坏性定量检测肝纤维化铁沉积的方法，铁沉积在TAA诱导的肝纤维化过程中可能起重要作用。
Liver fibrosis is the intermediate process and inevitable stage of the development of chronic liver disease into cirrhosis. Reducing the degree of liver fibrosis plays an extremely important role in treating chronic liver disease and preventing liver cirrhosis and liver cancer. The formation of liver fibrosis is affected by iron deposition to a certain extent, and excessive iron deposition further induces liver cirrhosis and liver cancer. Herein, confocal microbeam X-ray fluorescence (μ-XRF) was used to determine the intensity and biodistribution of iron deposition at different time points in the process of liver fibrosis induced by thioacetamide (TAA) in rats. To our best knowledge, this is the first study using confocal μ-XRF to analyze hepatic iron deposition in hepatic fibrosis. The results showed that there are minor and trace elements such as iron, potassium, and zinc in the liver of rats. Continuous injection of TAA solution resulted in increasing liver iron deposition over time. The intensity of iron deposition in liver tissue was also significantly reduced after bone mesenchymal stem cells (BMSCs) were injected. These findings indicated that confocal μ-XRF can be used as a nondestructive and quantitative method of evaluating hepatic iron deposition in hepatic fibrosis, and iron deposition may play an important role in the progression of hepatic fibrosis induced by TAA.
登录
查看更多内容
DOI：
10.1039/c2cp41858d
发表时间：
2012-10-21
期刊：
Physical chemistry chemical physics : PCCP
影响因子：
--
作者：
Chen H;Rogalski MM;Anker JN
通讯作者：
Anker JN
DOI：
10.26355/eurrev_202102_24870
发表时间：
2021-01-01
影响因子：
3.3
作者：
Chen, Z-K;Chen, D-Z;Chen, Y-P
通讯作者：
Chen, Y-P
利用共焦微 X 射线荧光对电解槽中稳态扩散中电极表面附近的离子分布进行空间分辨原位测量
影响因子：
7.4
作者：
Peng, Song;Liu, Zhiguo;Ding, Xunliang
通讯作者：
Ding, Xunliang
影响因子：
6.1
作者：
Mazel, Vincent;Reiche, Ina;Tchoreloff, Pierre
通讯作者：
Tchoreloff, Pierre
影响因子：
5
作者：
Alvarez-Marimon, Elena;Castillo-Michel, Hiram;Benseny-Cases, Nuria
通讯作者：
Benseny-Cases, Nuria