Protective efficacy of a Mycoplasma pneumoniae P1C DNA vaccine fused with the B subunit of Escherichia coli heat-labile enterotoxin.
Protective efficacy of a Mycoplasma pneumoniae P1C DNA vaccine fused with the B subunit of Escherichia coli heat-labile enterotoxin.
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与大肠杆菌不耐热肠毒素 B 亚基融合的肺炎支原体 P1C DNA 疫苗的保护功效。
DOI：
10.1139/w2012-051
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发表时间：
2012-05
期刊：
影响因子：
--
通讯作者：
胡四海
中科院分区：
文献类型：
--
作者：
胡四海
在本研究中，我们研究了将肺炎支原体P1蛋白羧基末端区（P1C）与大肠杆菌热不稳定毒素B亚基（LTB）融合构建的DNA疫苗的免疫调节反应。通过鼻内接种对照DNA、P1C DNA疫苗或LTB-P1C融合DNA疫苗对BALB/c小鼠进行免疫。抗m抗体的水平。与接种P1C DNA疫苗相比，接种LTB-P1C融合DNA疫苗可显著提高小鼠肺炎抗体及干扰素-γ和IL-4水平。LTB-P1C融合DNA疫苗可有效提高肺炎支原体特异性IgA和IgG水平。LTB和P1C融合小鼠支气管肺泡灌洗液和血清中IgG2a/IgG1比值明显高于单独接受P1C的小鼠。当小鼠鼻内感染10(7)CFU肺炎支原体菌株（M129）时，LTB-P1C融合DNA疫苗的保护作用明显优于P1C DNA疫苗（P < 0.05），结果表明，炎症反应较少，组织病理学评分值较低，肺炎支原体可检测数量较低，5 × 10(8) CFU肺炎支原体的攻击死亡率较低。这些结果表明，LTB-P1C融合DNA疫苗有效地提高了对肺炎支原体感染的保护作用，有效地减少了小鼠肺炎支原体的发展。
In the present study, we investigated the immunomodulatory responses of a DNA vaccine constructed by fusing Mycoplasma pneumoniae P1 protein carboxy terminal region (P1C) with the Escherichia coli heat-labile toxin B subunit (LTB). BALB/c mice were immunized by intranasal inoculation with control DNAs, the P1C DNA vaccine or the LTB-P1C fusion DNA vaccine. Levels of the anti-M. pneumoniae antibodies and levels of interferon-γ and IL-4 in mice were increased significantly upon inoculation of the LTB-P1C fusion DNA vaccine when compared with the inoculation with P1C DNA vaccine. The LTB-P1C fusion DNA vaccine efficiently enhanced the M. pneumoniae-specific IgA and IgG levels. The IgG2a/IgG1 ratio was significantly higher in bronchoalveolar lavages fluid and sera from mice fusion with LTB and P1C than mice receiving P1C alone. When the mice were challenged intranasally with 10(7) CFU M. pneumoniae strain (M129), the LTB-P1C fusion DNA vaccine conferred significantly better protection than P1C DNA vaccine (P < 0.05), as suggested by the results, such as less inflammation, lower histopathological score values, lower detectable number of M. pneumoniae strain, and lower mortality of challenging from 5 × 10(8) CFU M. pneumoniae. These results indicated that the LTB-P1C fusion DNA vaccine efficiently improved protective efficacy against M. pneumoniae infection and effectively attenuated development of M. pneumoniae in mice.
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