Role for ion transport in porcine vocal fold epithelial defense to acid challenge.
Role for ion transport in porcine vocal fold epithelial defense to acid challenge.
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DOI：
10.1177/0194599811428273
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发表时间：
2012-02
期刊：
影响因子：
--
通讯作者：
Sivasankar MP
中科院分区：
文献类型：
--
作者：
Erickson-Levendoski E;Sivasankar MP
在咽喉反流事件期间，声带上皮通常暴露于胃内容物，包括酸和胃蛋白酶。上皮细胞可能具有对反流的内在防御。本研究的第一个目的是研究声带上皮细胞离子转运是否是防御胃内容物的一种潜在机制。第二个目的是确定响应胃内容物的离子转运是否与碳酸氢盐的分泌相关。切除猪喉的前瞻性设计。实验室将猪声带（N = 56）的腔表面暴露于酸、胃蛋白酶或假激发。使用电生理技术测量基线和挑战暴露后的离子转运。为了检查特定的离子转运机制，声带用钠通道阻滞剂或碳酸氢盐通道阻滞剂进行预处理。在60秒的酸，但不是胃蛋白酶暴露，有一个显着增加离子转运。这种离子转运的快速增加是短暂的，与碳酸氢盐分泌有关。目前的数据表明，猪声带立即增加碳酸氢盐分泌后，暴露于酸。二头肌分泌可起到中和酸的作用。这些发现有助于识别声带防御反流的机制，并为反流引起的声带损伤的治疗提供了启示。
The vocal fold epithelium is routinely exposed to gastric contents, including acid and pepsin, during laryngopharyngeal reflux events. The epithelium may possess intrinsic defenses to reflux. The first objective of the current study was to examine whether vocal fold epithelial ion transport is one potential mechanism of defense to gastric contents. The second objective was to determine whether ion transport in response to gastric contents is associated with the secretion of bicarbonate. Prospective design in excised porcine larynges. Laboratory. Porcine vocal folds (N = 56) were exposed on the luminal surface to acid, pepsin, or sham challenges. Ion transport at baseline and following challenge exposure was measured using electrophysiological techniques. To examine specific ion transport mechanisms, vocal folds were pretreated with either a sodium channel blocker or bicarbonate channel blocker. Within 60 seconds of acid but not pepsin exposure, there was a significant increase in ion transport. This rapid increase in ion transport was transient and related to bicarbonate secretion. The current data suggest that porcine vocal folds immediately increase bicarbonate secretion following exposure to acid. Bicarbonate secretion may act to neutralize acid. These findings contribute to the identification of the mechanisms underlying vocal fold defense to reflux and offer implications for the development of treatments for reflux-induced vocal fold injury.
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影响因子：
2.6
作者：
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影响因子：
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DOI：
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