Actin dynamics in growth cones
Actin dynamics in growth cones
复制标题
生长锥中的肌动蛋白动力学
DOI：
--
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发表时间：
1991
影响因子：
5.3
通讯作者：
Nobutaka Hirokawa
中科院分区：
文献类型：
--
作者：
S. Okabe;Nobutaka Hirokawa
肌动蛋白掺入和营业额的神经生长锥的机制进行了研究，通过免疫电镜和低光水平的视频显微镜培养的神经元注射生物素标记的肌动蛋白或荧光标记的肌动蛋白。我们首先确定的网站肌动蛋白掺入生长锥的细胞骨架的免疫电子显微镜培养的神经元注射生物素标记的肌动蛋白，并与抗生物素抗体和金标记的二抗反应。注射后不久，生物素-肌动蛋白分子纳入细胞骨架被定位在远端部分的肌动蛋白束的丝状伪足和在膜相关的边缘的肌动蛋白丝网络。随着孵育时间的延长，生长锥中的大多数肌动蛋白聚合物被均匀地标记，这表明肌动蛋白亚基优先添加在预先存在的肌动蛋白丝的膜相关末端。然后，我们确定是否肌动蛋白丝易位内的生长锥低光水平的视频显微镜下的活神经元注射荧光标记的肌动蛋白和photobleached与激光束。当生长锥前缘的肌动蛋白荧光被漂白时，观察到漂白后的斑点向生长锥基部的向后易位。这一观察结果表明，在生长锥内存在向后流动的肌动蛋白聚合物。两者合计，这些结果表明，有一个连续的肌动蛋白单体在生长锥的前缘和连续向后易位的组装丝。
The mechanism of actin incorporation and turnover in the nerve growth cone was examined by immunoelectron microscopy and low-light-level video microscopy of cultured neurons injected with biotin-labeled actin or fluorescently labeled actin. We first determined the sites of actin incorporation into the cytoskeleton of growth cones by immunoelectron microscopy of cultured neurons injected with biotin-labeled actin and reacted with an anti-biotin antibody and a gold-labeled secondary antibody. Shortly after the injection, biotin-actin molecules incorporated into the cytoskeleton were localized in the distal part of actin bundles in the filopodia and at the membrane-associated fringe of the actin filament network. With longer incubation, most actin polymers in the growth cones were labeled uniformly, suggesting that actin subunits are added preferentially at the membrane-associated ends of preexisting actin filaments. We then determined whether actin filaments translocate within the growth cones by low-light-level video microscopy of living neurons injected with fluorescently labeled actin and photobleached with a laser beam. When actin fluorescence at the leading edge of a growth cone was bleached, a rearward translocation of the bleached spot toward the base of the growth cone was observed. This observation suggests the presence of a rearward flow of actin polymers within growth cones. Taken together, these results indicate that there is a continuous addition of actin monomers at the leading edge of the growth cone and a successive rearward translocation of the assembled filaments.
影响因子：
2.7
作者：
ROGERS, SL;LETOURNEAU, PC;FURCHT, LT
通讯作者：
FURCHT, LT
活果蝇胚胎中微管和肌动蛋白丝的行为。
DOI：
10.1242/dev.103.4.675
发表时间：
1988
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Kellogg,DR;Mitchison,TJ;Alberts,BM
通讯作者：
Alberts,BM
影响因子：
--
作者：
FISHER, GW;CONRAD, PA;TAYLOR, DL
通讯作者：
TAYLOR, DL