Preformed portals facilitate dendritic cell entry into afferent lymphatic vessels.
Preformed portals facilitate dendritic cell entry into afferent lymphatic vessels.
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DOI：
10.1084/jem.20091739
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发表时间：
2009-12-21
期刊：
影响因子：
--
通讯作者：
Sixt M
中科院分区：
文献类型：
--
作者：
Pflicke H;Sixt M
虽然这两个过程发生的速度相似，白细胞从血液循环外渗的研究，而内渗到淋巴管几乎没有研究。在一个共同的解释，即内部和外渗遵循类似的分子原理，我们以前表明，淋巴进入树突状细胞（DC）不需要整合素介导的粘附相互作用。在这项研究中，我们表明，DC进入也是独立的细胞周围的蛋白水解，提出了淋巴管是否提供预先存在的进入途径的问题。我们发现起始淋巴管的外淋巴基底膜是不连续的，因此留下了进入细胞的间隙。使用一种新开发的原位活细胞成像方法，使我们能够动态地可视化细胞及其细胞外环境，我们证明了DC通过这些不连续性进入，这些不连续性被传代细胞短暂地机械扩张。我们进一步表明，穿透底层淋巴管内皮层发生通过瓣阀缺乏连续的细胞间连接。总之，我们证明了自由细胞之间的沟通，淋巴管和淋巴腔。
Although both processes occur at similar rates, leukocyte extravasation from the blood circulation is well investigated, whereas intravasation into lymphatic vessels has hardly been studied. In contrast to a common assumption—that intra- and extravasation follow similar molecular principles—we previously showed that lymphatic entry of dendritic cells (DCs) does not require integrin-mediated adhesive interactions. In this study, we demonstrate that DC-entry is also independent of pericellular proteolysis, raising the question of whether lymphatic vessels offer preexisting entry routes. We find that the perilymphatic basement membrane of initial lymphatic vessels is discontinuous and therefore leaves gaps for entering cells. Using a newly developed in situ live cell imaging approach that allows us to dynamically visualize the cells and their extracellular environment, we demonstrate that DCs enter through these discontinuities, which are transiently mechanically dilated by the passaging cells. We further show that penetration of the underlying lymphatic endothelial layer occurs through flap valves lacking continuous intercellular junctions. Together, we demonstrate free cellular communication between interstitium and lymphatic lumen.
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炎症引起的白细胞跨淋巴管内皮迁移的机制。
影响因子：
15.3
作者：
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影响因子：
56.9
作者：
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影响因子：
6
作者：
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通讯作者：
Detmar, M
DOI：
10.1073/pnas.242401399
发表时间：
2002-12-10
影响因子：
11.1
作者：
Podgrabinska, S;Braun, P;Skobe, M
通讯作者：
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影响因子：
7.5
作者：
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