Growth hormone (GH) stimulates protein synthesis in cells transfected with GH receptor complementary DNA.-猫眼课题宝

生长激素 (GH) 刺激用 GH 受体互补 DNA 转染的细胞中的蛋白质合成。

DOI：

发表时间：

1990

期刊：

Molecular Endocrinology

影响因子：

通讯作者：

中科院分区：

医学2区

文献类型：

作者：

M. Emtner;L. Mathews;G. Norstedt

将含有大鼠GH受体cDNA的表达载体转染至中国仓鼠卵巢(CHO)细胞中，建立表达GH受体的稳定细胞系。与未转染的 CHO 细胞相比，转染细胞中 GH 受体的表达导致 GH 出现高亲和力 (Kd = 1.53 nM) 特异性结合。 [125I]hGH 与受体的交联以及随后的十二烷基硫酸钠 (SDS) 电泳给出了估计的受体摩尔重量为 84,000。在表达 GH 受体的 CHO 细胞中，GH 处理刺激的蛋白质合成比基础水平高 60%，但在受体阴性的亲本细胞中则不然。该效果仅在无血清条件下观察到，并且具有时间和剂量依赖性。这些结果表明，大鼠 GH 受体的异源表达导致 GH 特异性结合的出现和功能性 GH 反应的获得。

An expression vector containing a rat GH receptor cDNA was transfected into Chinese hamster ovary (CHO) cells, and stable cell lines expressing GH receptors were established. In contrast to nontransfected CHO cells, expression of GH receptors in transfected cells resulted in the appearance of high affinity (Kd = 1.53 nM) specific binding of GH. Cross-linking of [125I]hGH to the receptors and subsequent sodium dodecyl sulfate (SDS)-electrophoresis gave an estimated receptor mol wt of 84,000. GH treatment stimulated protein synthesis 60% over basal levels in GH receptor-expressing CHO cells, but not in the receptor-negative parental cells. The effect was observed only under serum-free conditions and was time and dose dependent. These results show that heterologous expression of the rat GH receptor results in the appearance of specific binding of GH and the acquisition of a functional GH response.